Protease Inhibitor Cocktail (EDTA-Free, 100× in DMSO)

Catalog No. C0001 Animal-derived-free(for general use)

TargetMol provides a wide array of customizable Protease Inhibitor Cocktails, each with varying mechanisms of actions, aimed at protecting your protein samples. They work by helping proteins avoid rapid degradation caused by endogenous proteases during the cell lysis and protein purification processes. The Protease Inhibitor Cocktail I contains six protease inhibitors with broad specificity, aimed at facilitating the inhibition of aspartyl, cysteine, serine, as well as several aminopeptidases.

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Protease Inhibitor Cocktail (EDTA-Free, 100× in DMSO)

パッケージサイズ	在庫状況	単価(税別)
1 mL	在庫あり	￥ 6,500
1 mL * 10	在庫あり	￥ 45,500
1 mL * 50	在庫あり	￥ 182,000
1 mL * 100	在庫あり	￥ 260,000

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Composition

Ingredient	CAS	MW	Concentration (100x)	Target	Type
AEBSF	30827-99-7	239.69	104 mM	serine proteases	Irreversible
Aprotinin	9087-70-1	6511.44	80 μM	serine proteases	Reversible
Bestatin	58970-76-6	308.37	5 mM	aminopeptidase B; LAP	Reversible
E-64	66701-25-5	357.41	1.5 mM	cysteine proteases	Irreversible
Leupeptin	55123-66-5	475.6	2 mM	cysteine proteases; serine proteases	Reversible
Pepstatin A	26305-03-3	685.89	1.5 mM	aspartic proteinase	Reversible

Features

Accurate Ingredients —— Accurate components and clear concentrations to avoid experimental uncertainty
Comprehensive Protection—— Effectively inhibit various proteases to protect proteins from being degraded
Cost-Effective —— Lower price with higher quality

(Reference: Neil D. Rawlings, Guy Salvesen et al. Handbook of Proteolytic Enzymes, Vol.1, 2012.)

Handling Instruction

Compatible with Western Blot analysis, Co-IP, pull-down, IF, IHC, kinase assay and etc.
Thaw in Room Temperature. Please centrifuge it at low speed before opening the cover, so as to throw the liquid adhered to the wall of the tube to the bottom.
Add concentrated cocktail at 1:100 (v/v) dilution to solution samples (such as cell lysates or tissue extracts) before assaying.
Briefly vortex cocktails to help facilitate the dissolution.
Extracts of up to 20 g wet weight.

Notice

Following initial thaw, aliquot and freeze (-20°C) to avoid thawing and refreezing repeatedly.
Some DUB proteases (one example is ATAXIN-3) cannot be suppressed by traditional protease inhibitors, such as E-64, AEBSF, bestatin, leupeptin and Aprotinin.

引用文献

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