keep away from direct sunlight
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
D-Luciferin potassium (D-Luciferin K Salt) is a popular bioluminescent substrate of luciferase in the presence of ATP.
パッケージサイズ | 在庫状況 | 単価(税別) | |||
---|---|---|---|---|---|
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10 mg | 在庫あり | ¥ 9,500 | |||
50 mg | 在庫あり | ¥ 17,000 | |||
100 mg | 在庫あり | ¥ 24,000 | |||
500 mg | 在庫あり | ¥ 48,000 | |||
1 g | 在庫あり | ¥ 67,000 |
説明 | D-Luciferin potassium (D-Luciferin K Salt) is a popular bioluminescent substrate of luciferase in the presence of ATP. |
In vitro |
METHODS: Preparation of D-Luciferin potassium for in vitro bioluminescence analysis: 1. Prepare 200× D-Luciferin reserve solution (30 mg/mL) with sterile water and mix gently until all dissolved. It can be used immediately after preparation or stored at -20℃. 2. Prepare 1× D-Luciferin (150 µg/mL) working solution in preheated tissue culture medium. 3. Aspirate the medium from the cultured cells. 4. Add 1× D-Luciferin solution to the cells before imaging. Incubate the cells at 37°C for a short period of time before imaging to increase signal. |
In vivo |
METHODS: Preparation of D-Luciferin potassium for in vivo bioluminescence analysis: 1. Prepare a fresh 15 mg/mL D-Luciferin stock solution in DPBS. Sterilize by filtering with 0.20 um filter. 2. Inject 10 µL/g of mouse body weight. 150 mg D-Luciferin/kg body weight per mouse. For example, for a mouse weighing 10 g, inject 100 µL to provide 1.5 mg D-Luciferin. 3. inject D-Luciferin intraperitoneally 10-15 min prior to imaging. fluorescein kinetic studies should be performed for eah animal model to determine peak signal times. |
キナーゼ試験 | Enolase activity assay is in the 2.0 mM MgSO4 and 400 mM KCl in the absence or presence of ENOblock or NaF, at 37°C by incubating pure enolase (3–9 U) in a buffer containing 50 mM imidazole-HCl (pH 6.8). The reaction is initiated by adding 1 μmol of 2-phospho-D-glycerate, and the OD is measured after 10 min of reaction time with a spectrophometer at 240 nm. |
別名 | D-Luciferin K Salt, D-Luciferin Potassium Salt |
分子量 | 318.41 |
分子式 | C11H7KN2O3S2 |
CAS No. | 115144-35-9 |
keep away from direct sunlight
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
DMSO: 2.63 mg/mL (8.26 mM), Sonication is recommended.
H2O: 50 mg/mL (157 mM), Sonication is recommended.
You can also refer to dose conversion for different animals. 詳細
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc.
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