keep away from direct sunlight
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
Sulforhodamine B sodium salt (Acid Red 52) is a fluorescent dye. It can be used from laser-induced fluorescence to the quantification of cellular proteins of cultured cells.
説明 | Sulforhodamine B sodium salt (Acid Red 52) is a fluorescent dye. It can be used from laser-induced fluorescence to the quantification of cellular proteins of cultured cells. |
In vitro | Sulforhodamine B (SRB) is often used as a membrane-impermeable polar tracer or used for cell density determination via the determination of cellular proteins (cytotoxicity assay). The SRB assay has been used to inexpensively conduct various screening assays to investigate cytotoxicity in cell-based studies. The protocol can be divided into four main steps: preparation of treatment, incubation of cells with treatment of choice, cell fixation, and SRB staining, and absorbance measurement. This assay is limited to manual or semiautomatic screening and can be used in an efficient and sensitive manner to test chemotherapeutic drugs or small molecules in adherent cells. It also has applications in evaluating the effects of gene expression modulation (knockdown, gene expression upregulation), as well as to study the effects of miRNA replacement on cell proliferation. |
別名 | Acid Red 52, Kiton Red 620 |
分子量 | 580.65 |
分子式 | C27H29N2NaO7S2 |
CAS No. | 3520-42-1 |
keep away from direct sunlight
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
DMSO: 50 mg/mL (86.11 mM), Sonication is recommended.
H2O: 20 mg/mL (34.44 mM), Sonication is recommended.
You can also refer to dose conversion for different animals. 詳細
bottom
Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc.
Sulforhodamine B sodium salt 3520-42-1 Others Inhibitor Sulforhodamine B Acid Red52 Acid Red 52 Kiton Red-620 Kiton Red 620 inhibit Kiton Red620 Sulforhodamine B sodium Acid Red-52 inhibitor