Powder: -20°C for 3 years | In solvent: -80°C for 1 year
Autophagy-IN-2 (Compound 7h) is an inhibitor of autophagic flux which induces cancer cell apoptosis and can be used for the research of triple-negative breast cancer [1].
パッケージサイズ | 在庫状況 | 単価(税別) |
---|---|---|
25 mg | 約6-8 週間 | ¥ 349,000 |
50 mg | 約6-8 週間 | ¥ 454,500 |
100 mg | 約6-8 週間 | ¥ 574,000 |
説明 | Autophagy-IN-2 (Compound 7h) is an inhibitor of autophagic flux which induces cancer cell apoptosis and can be used for the research of triple-negative breast cancer [1]. |
In vitro | Autophagy-IN-2 (Compound 7h), across a concentration range of 0-200 μM for 48 hours, exhibits anti-viability effects on various cancer cell lines, as evidenced by its IC 50 values across a panel including MDA-MB-231, MDA-MB-468, and others. At lower concentrations (0-20 μM) and varying time points up to 48 hours, this compound disrupts autophagic flux and DNA repair mechanisms by decreasing chromatin ubiquitination, linked to a p62-dependent process. Additionally, Autophagy-IN-2 triggers cell cycle arrest in the S-phase and initiates mitochondrial-dependent intrinsic apoptosis. Western blot analysis reveals a dose and time-dependent increase in LC3B-II, p62 accumulation, and DNA damage markers in treated TNBC cells, alongside modifications in cell cycle and apoptosis-related proteins. Cell cycle and apoptosis assays further confirm the compound's ability to induce S-phase arrest and significantly enhance apoptosis, particularly late-phase, in a dose-dependent manner in TNBC cells. |
In vivo | Autophagy-IN-2 (Compound 7h) administered intraperitoneally (i.p.) at doses of 5 and 15 mg/kg every three days for three weeks has been observed to dose-dependently suppress tumor growth in six-week-old female NOD/SCID mice with MDA-MB-231 xenografts. This treatment led to a dose-dependent inhibition of human TNBC tumor growth and induced a concentration-dependent increase in the expression of LC3B-II, p62, γH2AX, and PARP. |
分子量 | 309.37 |
分子式 | C17H19N5O |
CAS No. | 2755454-90-9 |
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
You can also refer to dose conversion for different animals. 詳細
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Autophagy-IN-2 2755454-90-9 Inhibitor inhibitor inhibit