store at low temperature,keep away from direct sunlight
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
BODIPY-Cholesterol (TF-Chol) is a cholesterol analog with a fluorescent BODIPY motif and cellular permeability, and is a novel BODIPY cholesterol probe.BODIPY-Cholesterol can be used to study intracellular sterol uptake and interorganelle sterol flux.
説明 | BODIPY-Cholesterol (TF-Chol) is a cholesterol analog with a fluorescent BODIPY motif and cellular permeability, and is a novel BODIPY cholesterol probe.BODIPY-Cholesterol can be used to study intracellular sterol uptake and interorganelle sterol flux. |
In vitro |
For the BODIPY-cholesterol assay:Reconstitute the analogue in DMSO to prepare a stock solution of BODIPY-cholesterol at a concentration of 1-10 μM[2]. Dilute cells with PBS (or other buffers), wash them (300×g, 10 min) to eliminate excess diluent, and resuspend the loose pellet with vehicle media to achieve the desired concentration[2].Label cell suspensions with BODIPY-cholesterol (final concentration of 0.5-5 μM), ensuring thorough mixing, and incubate for 10-20 minutes at 37 °C[1].Dilute the cells with each of the capacitation media conditions, as outlined in Incubation media, to a final concentration. Incubate for 2 hours before flow cytometric assessment[2]. |
別名 | BCh2 |
分子量 | 576.61 |
分子式 | C36H51BF2N2O |
CAS No. | 878557-19-8 |
store at low temperature,keep away from direct sunlight
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
DMSO: 1 mg/mL(1.73 mM), Sonication is recommended.
H2O: <0.1 mg/mL(Insoluble)
You can also refer to dose conversion for different animals. 詳細
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc.
BODIPY-Cholesterol 878557-19-8 BCh2 BODIPY Cholesterol BODIPYCholesterol Inhibitor inhibitor inhibit