Phosphatase Inhibitor Cocktail I (100× ddH2O)

Catalog No. C0002 Animal-derived-free(for general use)

It is easy for the proteins to be degraded or dephosphorylated during in vitro extraction procedure, which could cause an inaccurate result of protein expression detection. Therefore, adding protease inhibitors or phosphatase inhibitors into the extracts would be an effective method to prevent degradation and dephosphorylation of protein.

The phosphatase inhibitor mixture can effectively inhibit the dephosphorylation of common phosphatases on proteins and maintain the original phosphorylation state of proteins.

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Phosphatase Inhibitor Cocktail I (100× ddH2O)

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Composition

Ingredient	CAS	MW	Concentration (100x)	Target	Type
Sodium Fluoride	7681-49-4	41.99	100 mM	Acid Phosphatase	Reversible
Sodium Orthovanadate	13721-39-6	183.91	100 mM	Alkaline phosphatase, PTPs, ATPases	Reversible
Sodium Molybdate	7631-95-0	205.92	115 mM	Acid and phosphoprotein Phosphatase	Irreversible
Sodium Tartrate	868-18-8	194.05	400mM	Acid Phosphatase	Reversible
Imidazole	288-32-4	68.08	200 mM	Alkaline phosphatase	Reversible

Features

Accurate Ingredients —— Clearance of components and concentrations to avoid experimental uncertainty
Comprehensive Protection—— Effectively inhibit various proteases to protect proteins from being degraded
Cost-Effective —— Lower price with higher quality

Handling Instruction

Compatible with Western Blot analysis, IP, Co-IP, pull-down, IF, IHC, kinase assay and etc.
Add concentrated cocktail at 1:100 (v/v) dilution to samples solution (such as cell lysates or tissue extracts) before assaying.
Briefly vortex cocktails to help facilitate the dissolution.

Notice

Following initial thaw, aliquot and freeze (-20°C) to avoid thawing and refreezing repeatedly.
If Cocktail I and Cocktail II are used together, do not mix tube I with tube II beforehand, as there may be precipitation. To avoid this, please add them step by step during experiment.