15-PGDH Protein, Human, Recombinant (His) is expressed in E. coli expression system with His tag. The predicted molecular weight is 29.7 kDa and the accession number is P15428-1.
説明 | 15-PGDH Protein, Human, Recombinant (His) is expressed in E. coli expression system with His tag. The predicted molecular weight is 29.7 kDa and the accession number is P15428-1. |
Species | Human |
Expression Host | E. coli |
Tag | C-His |
Accession Number | P15428-1 |
別名 | PGDH1, hydroxyprostaglandin dehydrogenase 15-(NAD), PHOAR1, SDR36C1, 15-PGDH, PGDH |
Construction | The Human HPGD (NP_000851.2) (Met 1-Gln 266) was expressed, with a polyhistidine tag at the C-terminus. |
Protein Purity | > 92 % as determined by SDS-PAGE |
分子量 | 29.7 kDa (predicted) |
Endotoxin | < 1.0 EU/μg of the protein as determined by the LAL method. |
Formulation | Supplied as sterile 50 mM Tris, 100 mM NaCl, 0.5 mM DTT, 10% glycerol, pH 7.5. |
Reconstitution | A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information. |
Stability & Storage |
It is recommended to store the product under sterile conditions at -20℃ to -80℃. Samples are stable for up to 12 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
Shipping |
In general, Lyophilized powders are shipping with blue ice. Solutions are shipping with dry ice. |
Research Background | 15-hydroxyprostaglandin dehydrogenase [NAD+], also known as Prostaglandin dehydrogenase 1, HPGD, and PGDH1, is a member of the short-chain dehydrogenases/reductases (SDR) family. Prostaglandins (PGs) play a key role in the onset of labor in many species and regulate uterine contractility and cervical dilatation. Therefore, the regulation of prostaglandin output by PG synthesizing and metabolizing enzymes in the human myometrium may determine uterine activity patterns in human labor both at preterm and at term. Prostaglandin dehydrogenase (PGDH) metabolizes prostaglandins (PGs) to render them inactive. HPGD is down-regulated by cortisol, dexamethasone, and betamethasone and down-regulated in colon cancer. It is up-regulated by TGFB1. HPGD contributes to the regulation of events that are under the control of prostaglandin levels. HPGD catalyzes the NAD-dependent dehydrogenation of lipoxin A4 to form 15-oxo-lipoxin A4. and inhibits in vivo proliferation of colon cancer cells. Defects in HPGD are the cause of primary hypertrophic osteoarthropathy autosomal recessive (PHOAR), cranio-osteoarthropathy (COA), and isolated congenital nail clubbing. |
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15-PGDH Protein, Human, Recombinant (His) PGDH1 PGDH-1 hydroxyprostaglandin dehydrogenase 15-(NAD) PHOAR-1 PHOAR 1 PGDH 1 PHOAR1 SDR36C1 15-PGDH PGDH recombinant recombinant-proteins proteins protein