4-1BB Ligand/TNFSF9 Protein, Human, Recombinant (His) is expressed in E. coli expression system with C-6xHis tag. The predicted molecular weight is 16-20 KDa and the accession number is P41273.
パッケージサイズ | 在庫状況 | 単価(税別) |
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10 μg | 在庫あり | ¥ 19,000 |
50 μg | 約5 days | ¥ 57,500 |
500 μg | 約5 days | ¥ 280,000 |
1 mg | 約5 days | ¥ 626,500 |
生物学的情報 |
1. Loaded Human 4-1BB-Fc-His on Protein A Biosensor, can bind Human 4-1BBL-His with an affinity constant of 1.55 pM as determined in BLI assay. (Regularly tested)
2. Immobilized Human 4-1BBL-His at 10μg/ml (100 μl/well) can bind Human 4-1BB-Fc . The ED50 of Human 4-1BB-Fc is 16.8 ng/ml. (Regularly tested)
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説明 | 4-1BB Ligand/TNFSF9 Protein, Human, Recombinant (His) is expressed in E. coli expression system with C-6xHis tag. The predicted molecular weight is 16-20 KDa and the accession number is P41273. |
Species | Human |
Expression Host | E. coli |
Tag | C-6His |
Accession Number | P41273 |
別名 | 4-1BBL, Tumor necrosis factor ligand superfamily member 9, 4-1BB ligand, TNFSF9 |
Amino Acid | Arg71-Glu254 |
Construction | Recombinant Human 4-1BB Ligand is produced by our E.coli expression system and the target gene encoding Arg71-Glu254 is expressed with a 6His tag at the C-terminus. |
Protein Purity |
Greater than 95% as determined by reducing SDS-PAGE. Greater than 95% as determined by SEC-HPLC.
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分子量 | 16-20 KDa, reducing conditions |
Endotoxin | Less than 0.1 ng/µg (1 EU/µg) as determined by LAL test. |
Formulation | Lyophilized from a 0.2 μm filtered solution of 20mM Tris-HCl, 150mM NaCl, pH 7.4. |
Reconstitution | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
Stability & Storage |
Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
Shipping |
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature listed below. |
Research Background | Tumor necrosis factor ligand superfamily member 9(4-1BBL) is single-pass type II membrane protein which is a member of the the tumor necrosis factor family. 4-1BBL is a 254 amino acids cytokine that is expressed in brain, placenta, lung, skeletal muscle and kidney. TNFSF9 has been shown to reactivate anergic T lymphocytes in addition to promoting T lymphocyte proliferation. This cytokine may have a role in activation-induced cell death (AICD) and cognate interactions between T-cells and B-cells/macrophages. It has also been shown to be required for the optimal CD8 responses in CD8 T cells, and is thought to be involved in T cell-tumor cell interaction. |
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Please read the User Guide of Recombinant Proteins for more specific information.
4-1BB Ligand/TNFSF9 Protein, Human, Recombinant (His) TNFSF 9 4-1BBL Tumor necrosis factor ligand superfamily member 9 TNFSF-9 4-1BB ligand TNFSF9 recombinant recombinant-proteins proteins protein