Dengue virus (DENV) (type 1, strain US/Hawaii/1944) E/Envelope Protein (His) is expressed in Baculovirus insect cells with His tag. The predicted molecular weight is 12.4 kDa and the accession number is ACF49259.1.
| 説明 |
Dengue virus (DENV) (type 1, strain US/Hawaii/1944) E/Envelope Protein (His) is expressed in Baculovirus insect cells with His tag. The predicted molecular weight is 12.4 kDa and the accession number is ACF49259.1. |
| Species |
DENV
|
| Expression Host |
Baculovirus Insect Cells
|
| Tag |
C-His
|
| Accession Number |
ACF49259.1
|
| 別名 |
E Protein
|
| Construction |
The Dengue virus (DENV)(type 1, strain US/Hawaii/1944) E (ACF49259.1) (Val580-Lys680) was expressed with a polyhistidine tag at the C-terminus.
|
| Protein Purity |
> 95 % as determined by SDS-PAGE.
|
| 分子量 |
12.4 kDa (predicted)
|
| Endotoxin |
< 1.0 EU/μg of the protein as determined by the LAL method.
|
| Formulation |
Lyophilized from a solution filtered through a 0.22 μm filter, containing 20 mM Tris, 500 mM NaCl, pH 8.0, 10% glycerol. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
|
| Reconstitution |
A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
|
| Stability & Storage |
It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted protein solutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
|
| Shipping |
In general, Lyophilized powders are shipping with blue ice.
|
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