Dengue virus (DENV)(type 3, strain Philippines/H87/1956) E/Envelope Protein (aa 247-675, His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 48.79 kDa and the accession number is AAA99437.1.
| 生物学的情報 |
Testing in progress
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| 説明 |
Dengue virus (DENV)(type 3, strain Philippines/H87/1956) E/Envelope Protein (aa 247-675, His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 48.79 kDa and the accession number is AAA99437.1. |
| Species |
DENV
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| Expression Host |
HEK293 Cells
|
| Tag |
C-His
|
| Accession Number |
AAA99437.1
|
| 別名 |
E Protein
|
| Construction |
The Dengue virus (DENV)(type 3, strain Philippines/H87/1956) Envelope Protein (AAA99437.1) (Phe247-Ser675) was expressed with a polyhistidine tag at the C-terminus.
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| Protein Purity |
> 95 % as determined by SDS-PAGE.
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| 分子量 |
48.79 kDa (predicted)
|
| Endotoxin |
< 1.0 EU/μg of the protein as determined by the LAL method.
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| Formulation |
Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
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| Reconstitution |
A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
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| Stability & Storage |
It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted proteinsolutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
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| Shipping |
In general, Lyophilized powders are shipping with blue ice.
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