Influenza A H1N1 (A/Puerto Rico/8/34/Mount Sinai) Matrix protein 1/M1 Protein (His) is expressed in E. coli expression system with His tag. The predicted molecular weight is 29.3 kDa and the accession number is B4UPA8.
| 生物学的情報 |
Testing in progress
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| 説明 |
Influenza A H1N1 (A/Puerto Rico/8/34/Mount Sinai) Matrix protein 1/M1 Protein (His) is expressed in E. coli expression system with His tag. The predicted molecular weight is 29.3 kDa and the accession number is B4UPA8. |
| Species |
H1N1
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| Expression Host |
E. coli
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| Tag |
His
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| Accession Number |
B4UPA8
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| 別名 |
cholinergic receptor, muscarinic 1
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| Construction |
A DNA sequence encoding the influenza A H1N1 Virus (A/Puerto Rico/8/34/Mount Sinai) M1 protein (NP_040978.1) (Ser 2-Lys 252) was expressed, with a polyhistidine tag at the N-terminus.
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| Protein Purity |
> 90 % as determined by SDS-PAGE
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| 分子量 |
29.3 kDa (predicted)
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| Endotoxin |
Please contact us for more information.
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| Formulation |
Lyophilized from sterile 20mM tris, 0. 5mM DTT, 0. 5mM EDTA, 5% glycerol, 50mM NaCl, pH 7.6Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0. 01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
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| Reconstitution |
A hardcopy of datasheet with reconstitution instructions is sent along with the products. Please refer to it for detailed information.
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| Stability & Storage |
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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| Shipping |
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
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| Research Background |
The Influenza virus matrix protein 1 (M1) is a matrix protein of the influenza virus. M1 protein has been shown to play a crucial role in virus replication, assembly, and budding. It forms a coat inside the viral envelope. This is a bifunctional membrane/RNA-binding protein that mediates the encapsidation of RNA-nucleoprotein cores into the membrane envelope. M1 consists of two domains connected by a linker sequence. The N-terminal domain has a multi-helical structure. The C-terminal domain also contains an alpha-helical structure. The M1 protein is the most abundant structural protein in influenza A virus particles. M1 protein of the influenza A virus plays multiple roles in virion assembly and infection. M1 protein was a candidate antigen for a broad-spectrum influenza virus vaccine and the adjuvant chitosan significantly improved the efficacy of the M1 vaccine.
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