Influenza A H1N1 (A/California/04/2009) Hemagglutinin/HA-specific B cell probe (His)

Influenza A H1N1 (A/California/04/2009) Hemagglutinin/HA-specific B cell probe (His) is expressed in Baculovirus insect cells with His tag. The predicted molecular weight is 57.8 kDa and the accession number is C3W5S1.

Influenza A H1N1 (A/California/04/2009) Hemagglutinin/HA-specific B cell probe (His)

パッケージサイズ	在庫状況	単価(税別)
100 μg	在庫あり	￥ 103,500
200 μg	約5 days	￥ 177,000
500 μg	約5 days	￥ 358,000

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生物学的情報	Testing in progress
説明	Influenza A H1N1 (A/California/04/2009) Hemagglutinin/HA-specific B cell probe (His) is expressed in Baculovirus insect cells with His tag. The predicted molecular weight is 57.8 kDa and the accession number is C3W5S1.

Species	H1N1
Expression Host	Baculovirus-Insect Cells
Tag	His
Accession Number	C3W5S1
別名	Harvey rat sarcoma viral oncogene homolog
Construction	A DNA sequence encoding the Influenza A virus (A/California/04/2009 (H1N1)) hemagglutinin (ACP41105.1) (Met1-Gly519, with a mutation Tyr 108 Phe), termed as HA, was expressed with a polyhistidine tag at the C-terminus.
Protein Purity	> 95 % as determined by SDS-PAGE.
分子量	57.8 kDa (predicted)

Endotoxin	< 1.0 EU per μg of the protein as determined by the LAL method.
Formulation	Lyophilized from sterile 20 mM Tris, 500 mM NaCl, pH 8.0. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0. 01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
Reconstitution	A hardcopy of datasheet with reconstitution instructions is sent along with the products. Please refer to it for detailed information.
Stability & Storage	Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Shipping	In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Research Background	The influenza viral Hemagglutinin (HA) protein is a homotrimer with a receptor binding pocket on the globular head of each monomer.HA has at least 18 different antigens. These subtypes are named H1 through H18.HA has two functions. Firstly, it allows the recognition of target vertebrate cells, accomplished through the binding to these cells' sialic acid-containing receptors. Secondly, once bound it facilitates the entry of the viral genome into the target cells by causing the fusion of the host endosomal membrane with the viral membrane. The influenza virus Hemagglutinin (HA) protein is translated in cells as a single protein, HA, or hemagglutinin precursor protein. For viral activation, hemagglutinin precursor protein (HA) must be cleaved by a trypsin-like serine endoprotease at a specific site, normally coded for by a single basic amino acid (usually arginine) between the HA1 and HA2 domains of the protein. After cleavage, the two disulfide-bonded protein domains produce the mature form of the protein subunits as a prerequisite for the conformational change necessary for fusion and hence viral infectivity.

参考文献

1. White JM, Hoffman LR, Arevalo JH, et al. Attachment and entry of influenza virus into host cells. Pivotal roles of hemagglutinin. In Chiu W, Burnett RM, Garcea RL. Structural Biology of Viruses.1997Suzuki Y.Sialobiology of influenza: molecular mechanism of host range variation of influenza viruses. Biol. Pharm. Bull. 2005. 2. Senne DA, Panigrahy B, Kawaoka Y, et al. Survey of the hemagglutinin (HA) cleavage site sequence of H5 and H7 avian influenza viruses: amino acid sequence at the HA cleavage site as a marker of pathogenicity potential. Avian Dis. 1996Donald J. Benton,Influenza hemagglutinin membrane anchor,PNAS,2018

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Influenza A H1N1 (A/California/04/2009) Hemagglutinin/HA-specific B cell probe (His)

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