Nucleocapsid protein (N) is the major viral structural component; its main function is to protect and encapsidate the viral RNA forming viral RNP complex. It is encoded by the S segment vRNA and is abundantly expressed in the cytoplasm of infected cells. SARS-COV-2 Nucleocapsid Protein CTD Domain (His & Avi) is expressed in E. coli expression system with N-His-Avi tag. The predicted molecular weight is 17 kDa and the accession number is P0DTC9.
| 説明 |
Nucleocapsid protein (N) is the major viral structural component; its main function is to protect and encapsidate the viral RNA forming viral RNP complex. It is encoded by the S segment vRNA and is abundantly expressed in the cytoplasm of infected cells. SARS-COV-2 Nucleocapsid Protein CTD Domain (His & Avi) is expressed in E. coli expression system with N-His-Avi tag. The predicted molecular weight is 17 kDa and the accession number is P0DTC9. |
| Species |
SARS-CoV-2
|
| Expression Host |
E. coli
|
| Tag |
N-His-Avi
|
| Accession Number |
P0DTC9
|
| 別名 |
Nucleocapsid, NP, NC
|
| Construction |
Gly243-Pro364
|
| Protein Purity |
> 95% as determined by Tris-Bis PAGE
|
| 分子量 |
17 kDa (predicted) same as Tris-Bis PAGE result.
|
| Endotoxin |
< 1 EU/μg by the LAL method.
|
| Formulation |
Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS (pH 7.4). Typically, 8% trehalose is incorporated as a protective agent before lyophilization.
|
| Reconstitution |
Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.
|
| Stability & Storage |
It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted protein solutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
|
| Shipping |
In general, Lyophilized powders are shipping with blue ice.
|
| Research Background |
Nucleocapsid protein (N) is the major viral structural component; its main function is to protect and encapsidate the viral RNA forming viral RNP complex. It is encoded by the S segment vRNA and is abundantly expressed in the cytoplasm of infected cells.
|
参考文献
Calculator
Reconstitution Calculator
Recombinant Proteins Dilute Calculator
Specific Activity Calculator
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