SARS-CoV-2 Plpro/papain-like protease Protein (aa 1564-1880, His)

SARS-CoV-2 Plpro/papain-like protease Protein (aa 1564-1880, His) is expressed in E. coli expression system. The predicted molecular weight is 36.79 kDa and the accession number is QVD65746.1.

SARS-CoV-2 Plpro/papain-like protease Protein (aa 1564-1880, His)

パッケージサイズ	在庫状況	単価(税別)
100 μg	約5 days	￥ 160,500

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説明	SARS-CoV-2 Plpro/papain-like protease Protein (aa 1564-1880, His) is expressed in E. coli expression system. The predicted molecular weight is 36.79 kDa and the accession number is QVD65746.1.

Species	SARS-CoV-2
Expression Host	E. coli
Tag	Tag Free
Accession Number	QVD65746.1
Construction	A DNA sequence encoding the SARS-CoV-2 (2019-nCoV) papain-like protease (QVD65746.1) (Glu1564-Val1880) was expressed with a polyhistidine tag at the N-terminus.
Protein Purity	> 90 % as determined by SDS-PAGE.
分子量	36.79 kDa (predicted)

Endotoxin	Please contact us for more information.
Formulation	Lyophilized from sterile 20mM Tris 500mM NaCl, pH 7.4. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0. 01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
Reconstitution	A hardcopy of datasheet with reconstitution instructions is sent along with the products. Please refer to it for detailed information.
Stability & Storage	Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Shipping	In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Research Background	The coronaviral proteases, papain-like protease (PLpro) and 3C-like protease (3CLpro), are attractive antiviral drug targets because they are essential for coronaviral replication. PLpro has the additional function of stripping ubiquitin and ISG15 from host-cell proteins to aid coronaviruses in their evasion of the host innate immune responses. Targeting PLpro with antiviral drugs may have an advantage in not only inhibiting viral replication but also inhibiting the dysregulation of signaling cascades in infected cells that may lead to cell death in surrounding, uninfected cells.

参考文献

1. Yahira M. Báez-Santos. The SARS-coronavirus papain-like protease: Structure, function and inhibition by designed antiviral compounds. Antiviral Research.2015

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SARS-CoV-2 Plpro/papain-like protease Protein (aa 1564-1880, His)

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