Zika virus (ZIKV) (strain Zika SPH2016) ZIKV-E (Stem/anchor domain of flavivirus envelope glycoprotein E) protein (Fc)

Zika virus (ZIKV) (strain Zika SPH2016) ZIKV-E (Stem/anchor domain of flavivirus envelope glycoprotein E) protein (Fc) is expressed in HEK293 mammalian cells with Fc tag. The predicted molecular weight is 36.7 kDa and the accession number is ALU33341.1.

Zika virus (ZIKV) (strain Zika SPH2016) ZIKV-E (Stem/anchor domain of flavivirus envelope glycoprotein E) protein (Fc)

パッケージサイズ	在庫状況	単価(税別)
100 μg	約5 days	￥ 161,000

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説明	Zika virus (ZIKV) (strain Zika SPH2016) ZIKV-E (Stem/anchor domain of flavivirus envelope glycoprotein E) protein (Fc) is expressed in HEK293 mammalian cells with Fc tag. The predicted molecular weight is 36.7 kDa and the accession number is ALU33341.1.

Species	ZIKV
Expression Host	HEK293 Cells
Tag	C-Fc
Accession Number	ALU33341.1
Construction	The Zika virus (strain Zika SPH2016) E_stem (Stem/anchor domain of flavivirus envelope glycoprotein E) (ALU33341.1) (Gly698-Ala794) was expressed with the Fc region of Human IgG1 at the C-terminus.
Protein Purity	> 95 % as determined by SDS-PAGE.
分子量	36.7 kDa (predicted)

Endotoxin	< 1.0 EU/μg of the protein as determined by the LAL method.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
Reconstitution	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
Stability & Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted proteinsolutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
Shipping	In general, Lyophilized powders are shipping with blue ice.
Research Background	Envelope of Zika virus is responsible for receptor binding and membrane. Analysis of the envelope protein of Zika, from Brazilian Zika SPH215 (KU321639), indicates predicted B and T cell epitopes in peptides that are consistent with those reported for dengue, YFYF and Japanese encephalitis. The envelope Domain II B cell epitope, to which much dengue non-neutralizing cross-reaction is attributed, is also conserved also in Zika virus, consistent with prior field observations of cross-reactivity with dengue and YF.Domain III of the Zika envelope protein, likely the main specific neutralizing domain, is distinct from recent Brazilian dengue isolates and a recent Peruvian YF isolate (GQ379163), 76% of possible major histocompatibility complex class (MHC) I and MHC II binding peptides and potential B cell linear epitopes are unique to Zika virus.

参考文献

1. Basarab M, et al. Zika virus. BMJ. 2016 Feb 26;352:i1049. 2. Júnior VL, et al. Zika virus: a review to clinicians, Acta Med Port 2015 Nov-Dec;28(6):760-765L. 3. Zammarchi, et al.Zika virus infection in a traveler returning to Europe from Brazil, March 2015. 4. Arturo Galindo-Fraga, et al. Zika virus: A new epidemic on our doorstep. Rev Inves Clin.2015, 67: 329-32.

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Zika virus (ZIKV) (strain Zika SPH2016) ZIKV-E (Stem/anchor domain of flavivirus envelope glycoprotein E) protein (Fc)

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