AKT3 Protein, Human, Recombinant (GST) is expressed in Baculovirus insect cells with GST tag. The predicted molecular weight is 81 kDa and the accession number is Q9Y243-1.
説明 | AKT3 Protein, Human, Recombinant (GST) is expressed in Baculovirus insect cells with GST tag. The predicted molecular weight is 81 kDa and the accession number is Q9Y243-1. |
Species | Human |
Expression Host | Baculovirus Insect Cells |
Tag | N-GST |
Accession Number | Q9Y243-1 |
別名 | PRKBG, RAC-γ, RAC-PK-γ, MPPH, PKB-γ, PKBG, RAC-PK-gamma, STK-2, PKB-GAMMA, RAC-gamma, v-akt murine thymoma viral oncogene homolog 3, MPPH2 |
Construction | The full length of Human PKBG (NP_005456.1) (Met 1-Glu 479) |
Protein Purity | > 90 % as determined by SDS-PAGE |
分子量 | 81 kDa (predicted) |
Endotoxin | < 1.0 EU/μg of the protein as determined by the LAL method. |
Formulation | Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization. |
Reconstitution | A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information. |
Stability & Storage |
It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted proteinsolutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
Shipping |
In general, Lyophilized powders are shipping with blue ice. |
Research Background | v-akt murine thymoma viral oncogene homolog 3 (AKT3), also known as PKB-GAMMA, with AKT1/PKBalpha, AKT2/PKBbeta, are the memerbers of Akt kinase family, share extensive structural similarity and perform common as well as unique functions within cells. The Akt signaling cascade initiates at the cell surface when growth factors or other extracellular stimuli activate phosphoinositide 3-kinase (PI3K). AKT3 was discovered to be the predominant isoform activated in sporadic melanomas. Levels of activity increased during melanoma progression with metastatic melanomas having the highest activity. Although mechanisms of AKT3 activation remain to be fully characterized, overexpression of AKT3 and decreased PTEN activity play important roles in this process. Targeted reduction of AKT3 activity decreased survival of melanoma tumor cells leading to inhibition of tumor development, which may be therapeutically effective for shrinking tumors in melanoma patients. AKT2 and AKT3 play an important role in the viability of human malignant glioma cells. Targeting AKT2 and AKT3 may hold promise for the treatment of patients with gliomas. |
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Please read the User Guide of Recombinant Proteins for more specific information.
AKT3 Protein, Human, Recombinant (GST) MPPH 2 PRKBG RAC-γ RAC-PK-γ MPPH STK 2 PKB-γ PKBG STK2 RAC-PK-gamma MPPH-2 STK-2 PKB-GAMMA RAC-gamma v-akt murine thymoma viral oncogene homolog 3 MPPH2 recombinant recombinant-proteins proteins protein