AKT3 Protein, Mouse, Recombinant (aa 106-479, His & GST)

AKT3 Protein, Mouse, Recombinant (aa 106-479, His & GST) is expressed in Baculovirus insect cells with His and GST tag. The predicted molecular weight is 71 kDa and the accession number is Q9WUA6-1.

AKT3 Protein, Mouse, Recombinant (aa 106-479, His & GST)

パッケージサイズ	在庫状況	単価(税別)
50 μg	約5 days	￥ 114,500

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説明	AKT3 Protein, Mouse, Recombinant (aa 106-479, His & GST) is expressed in Baculovirus insect cells with His and GST tag. The predicted molecular weight is 71 kDa and the accession number is Q9WUA6-1.

Species	Mouse
Expression Host	Baculovirus Insect Cells
Tag	His, GST
Accession Number	Q9WUA6-1
別名	v-akt murine thymoma viral oncogene homolog 3, Nmf350, D930002M15Rik, AI851531
Construction	The Mouse AKT3 (Q9WUA6-1) (Ala106-Glu479) was expressed with the N-terminal polyhistidine-tagged GST tag at the N-terminus.
Protein Purity	> 90 % as determined by SDS-PAGE
分子量	71 kDa (predicted)

Endotoxin	< 1.0 EU/μg of the protein as determined by the LAL method.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing 20 mM Tris, 500 mM Nacl, pH 7.4, 10% glycerol. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
Reconstitution	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
Stability & Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted proteinsolutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
Shipping	In general, Lyophilized powders are shipping with blue ice.
Research Background	v-akt murine thymoma viral oncogene homolog 3 (AKT3), also known as PKB-GAMMA, with AKT1/PKBalpha, AKT2/PKBbeta, are the memerbers of Akt kinase family, share extensive structural similarity and perform common as well as unique functions within cells. The Akt signaling cascade initiates at the cell surface when growth factors or other extracellular stimuli activate phosphoinositide 3-kinase (PI3K). AKT3 was discovered to be the predominant isoform activated in sporadic melanomas. Levels of activity increased during melanoma progression with metastatic melanomas having the highest activity. Although mechanisms of AKT3 activation remain to be fully characterized, overexpression of AKT3 and decreased PTEN activity play important roles in this process. Targeted reduction of AKT3 activity decreased survival of melanoma tumor cells leading to inhibition of tumor development, which may be therapeutically effective for shrinking tumors in melanoma patients. AKT2 and AKT3 play an important role in the viability of human malignant glioma cells. Targeting AKT2 and AKT3 may hold promise for the treatment of patients with gliomas.

参考文献

1. Mure H,et al.(2010) Akt2 and Akt3 play a pivotal role in malignant gliomas. Neuro Oncol. 12(3): 221-32.Koseoglu S,et al.(2007) AKT1, AKT2 and AKT3-dependent cell survival is cell line-specific and knockdown of all three isoforms selectively induces apoptosis in 20 human tumor cell lines. Cancer Biol Ther. 6(5): 755-62. 2. Cristiano BE,et al.(2006) A specific role for AKT3 in the genesis of ovarian cancer through modulation of G(2)-M phase transition. Cancer Res. 66(24): 11718-25. 3. Robertson GP. (2005) Functional and therapeutic significance of Akt deregulation in malignant melanoma. Cancer Metastasis Rev. 24(2): 273-85. 4. Altomare DA,et al.(2005) Perturbations of the AKT signaling pathway in human cancer. Oncogene. 24: 7455-64.Stahl JM,et al.(2004) Deregulated Akt3 activity promotes development of malignant melanoma. Cancer Res. 64: 7002-10.

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AKT3 Protein, Mouse, Recombinant (aa 106-479, His & GST)

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