ALDH4A1 Protein, Human, Recombinant

ALDH4A1 Protein, Human, Recombinant is expressed in Baculovirus insect cells. The predicted molecular weight is 59.2 kDa and the accession number is AAH07581.1.

ALDH4A1 Protein, Human, Recombinant

パッケージサイズ	在庫状況	単価(税別)
100 μg	約5 days	￥ 184,000

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説明	ALDH4A1 Protein, Human, Recombinant is expressed in Baculovirus insect cells. The predicted molecular weight is 59.2 kDa and the accession number is AAH07581.1.

Species	Human
Expression Host	Baculovirus Insect Cells
Tag	Tag Free
Accession Number	AAH07581.1
別名	aldehyde dehydrogenase 4 family, member A1, P5CD, P5CDh, ALDH4
Construction	The Human ALDH4A1 (AAH07581.1) (Lys 25-Gln 563) was expressed and purified with two additional amino acids (Gly & Pro ) at the N-terminus.
Protein Purity	> 95 % as determined by SDS-PAGE
分子量	59.2 kDa (predicted)

Endotoxin	< 1.0 EU/μg of the protein as determined by the LAL method.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing 20 mM Tris, 500 mM NaCl, 10% glycerol, pH 8.5. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
Reconstitution	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
Stability & Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted proteinsolutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
Shipping	In general, Lyophilized powders are shipping with blue ice.
Research Background	ALDH4A1 is a member of the aldehyde dehydrogenase family. Aldehyde dehydrogenase enzymes function in the metabolism of many molecules including certain fats (cholesterol and other fatty acids) and protein building blocks (amino acids). Additional aldehyde dehydrogenase enzymes detoxify external substances, such as alcohol and pollutants, and internal substances, such as toxins that are formed within cells. ALDH4A1 is expressed abundantly in liver followed by skeletal muscle, kidney, heart, brain, placenta, lung and pancreas. It is a mitochondrial matrix NAD-dependent dehydrogenase which catalyzes the second step of the proline degradation pathway, converting pyrroline-5-carboxylate to glutamate. Defects in ALDH4A1 are the cause of hyperprolinemia type 2 (HP-2). HP-2 is characterized by the accumulation of delta-1-pyrroline-5-carboxylate (P5C) and proline. The disorder may be causally related to neurologic manifestations, including seizures and mental retardation.

参考文献

1. Goodman SI, et al. (1974) Defective hydroxyproline metabolism in type II hyperprolinemia. Biochemical medicine. 10 (4): 329-36. 2. Maruyama K, et al. (1994) Oligo-capping: a simple method to replace the cap structure of eukaryotic mRNAs with oligoribonucleotides. Gene. 138 (1-2): 171-4. 3. Vasiliou V, et al. (2005) Analysis and update of the human aldehyde dehydrogenase (ALDH) gene family. Hum Genomics. 2 (2): 138-43.

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ALDH4A1 Protein, Human, Recombinant

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