ANGPT2/Angiopoietin-2 Protein, Human, Recombinant (mFc)

ANGPT2/Angiopoietin-2 Protein, Human, Recombinant (mFc) is expressed in HEK293 mammalian cells with mFc tag. The predicted molecular weight is 80.3 kDa and the accession number is O15123-1.

ANGPT2/Angiopoietin-2 Protein, Human, Recombinant (mFc)

パッケージサイズ	在庫状況	単価(税別)
50 μg	約5 days	￥ 70,500
500 μg	約5 days	￥ 459,000

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説明	ANGPT2/Angiopoietin-2 Protein, Human, Recombinant (mFc) is expressed in HEK293 mammalian cells with mFc tag. The predicted molecular weight is 80.3 kDa and the accession number is O15123-1.

Species	Human
Expression Host	HEK293 Cells
Tag	C-mFc
Accession Number	O15123-1
別名	ANG2, AGPT2, Angiopoietin-2, angiopoietin 2
Construction	The Human ANGPT2 (NP_001138.1) (Met1-Gln248 and Lys275-Phe496 linked by a polypeptide linker) was expressed with the Fc region of Mouse IgG2a at the C-terminus.
Protein Purity	> 85 % as determined by SDS-PAGE.
分子量	80.3 kDa (predicted)

Endotoxin	< 1.0 EU/μg of the protein as determined by the LAL method.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
Reconstitution	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
Stability & Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted proteinsolutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
Shipping	In general, Lyophilized powders are shipping with blue ice.
Research Background	Angiopoietin-2 (ANG 2, or ANGPT2), is a member of the ANG family, which plays an important role in angiogenesis during the development and growth of human cancers. Both ANGPT-1 and ANGPT-2 appear to bind to the tyrosine kinase receptor, Tie-2, found primarily on the luminal surface of endothelial cells. ANG-2's role in angiogenesis generally is considered as an antagonist for ANG1, inhibiting ANG1-promoted Tie2 signaling, which is critical for blood vessel maturation and stabilization. ANG-2 modulates angiogenesis in a cooperative manner with another important angiogenic factor, vascular endothelial growth factor A. Genetic studies have revealed that ANG-2 also is critical in lymphangiogenesis during development. ANG-2 has multiple physiologic effects that regulate vascular tone, hormone secretion, tissue growth and neural activity. Several reports indicate that ANG-2 can induce neovascularization in experimental systems due to the expression of different growth factors such as angiopoietin 2, vascular endothelial factor, and its receptor, fibroblast growth factor, platelet derived growth factor, transforming growth factor beta and epidermal growth factor. In addition, ANG-2 is strongly expressed in the vasculature of many tumors and it has been suggested that ANG-2 may act synergistically with other cytokines such as vascular endothelial growth factor to promote tumor-associated Angiogenesis and tumor progression.

参考文献

1. Thomas M,et al.(2009) The role of the Angiopoietins in vascular morphogenesis. Angiogenesis. 12(2): 125-37. 2. Hu B,et al.(2009) Angiopoietin-2: development of inhibitors for cancer therapy. Curr Oncol Rep. 11(2): 111-6. 3. Fiedler U,et al.(2006) Angiopoietins: a link between angiogenesis and inflammation. Trends Immunol. 27: 552-8. 4. Escobar E,et al.(2004) Angiotensin II, cell proliferation and angiogenesis regulator: biologic and therapeutic implications in cancer. Curr Vasc Pharmacol. 2(4): 385-99.

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ANGPT2/Angiopoietin-2 Protein, Human, Recombinant (mFc)

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