ANXA2 Protein, Mouse, Recombinant (His)

ANXA2 Protein, Mouse, Recombinant (His) is expressed in E. coli expression system with N-His tag. The predicted molecular weight is 39.6 kDa and the accession number is P07356.

ANXA2 Protein, Mouse, Recombinant (His)

パッケージサイズ	在庫状況	単価(税別)
100 μg	在庫あり	￥ 96,000
500 μg	約5 days	￥ 383,500
1 mg	約5 days	￥ 643,000

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説明	ANXA2 Protein, Mouse, Recombinant (His) is expressed in E. coli expression system with N-His tag. The predicted molecular weight is 39.6 kDa and the accession number is P07356.

Species	Mouse
Expression Host	E. coli
Tag	N-His
Accession Number	P07356
別名	LIP2PAP-IV, Annexin II, LPC2DP36, LPC2D, CAL1H, Annexin A2, ANX2L4, ANX2, PAP-IV, p36, Lipocortin II, Chromobindin-8, ANXA2
Construction	Ser2-Asp339
Protein Purity	> 95% as determined by Tris-Bis PAGE
分子量	39.6 kDa (predicted) same as Tris-Bis PAGE result.

Endotoxin	< 1 EU/μg by the LAL method.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing 20 mM MES, 150 mM NaCl, 200 mM L-arginine, 5 mM DTT (pH 5.5). Typically, 8% trehalose is incorporated as a protective agent before lyophilization.
Reconstitution	Reconstitute the lyophilized protein in 20mM MES, 150mM NaCl, 200mM L-arginine, 5mM DTT (pH 5.5). The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.
Stability & Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted proteinsolutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
Shipping	In general, Lyophilized powders are shipping with blue ice.
Research Background	ANXA2, highly expressed in invasive breast cancer cells, is closely related with poor prognosis, and acts as a molecular switch to EGFR activation. ANXA2 expression is inversely correlated with cell sensitivity to gefitinib. Knockdown of ANXA2 expression in MDA-MB-231 cells increased the gefitinib induced cell death. When ANXA2 was overexpressed in MCF7 cells, the gefitinib induced cell death was decreased. Furthermore, the phosphorylation of ANXA2 at Tyr23 is negatively correlated with the sensitivity of TNBC to gefitinib.

参考文献

1. Zhang Y, et al. ANXA2 could act as a moderator of EGFR-directed therapy resistance in triple negative breast cancer. Biosci Biotechnol Biochem. 2018 Oct;82(10):1733-174doi: 10.1080/09168451.2018.1484275. Epub 2018 Jun 18. PMID: 29912633.

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ANXA2 Protein, Mouse, Recombinant (His)

参考文献

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