APCDD1 Protein, Mouse, Recombinant (His) is expressed in HEK293 mammalian cells with C-His tag. The predicted molecular weight is 54.4 kDa and the accession number is Q3U128.
パッケージサイズ | 在庫状況 | 単価(税別) |
---|---|---|
100 μg | 約5 days | ¥ 112,000 |
500 μg | 約5 days | ¥ 447,500 |
1 mg | 約5 days | ¥ 746,000 |
説明 | APCDD1 Protein, Mouse, Recombinant (His) is expressed in HEK293 mammalian cells with C-His tag. The predicted molecular weight is 54.4 kDa and the accession number is Q3U128. |
Species | Mouse |
Expression Host | HEK293 Cells |
Tag | C-His |
Accession Number | Q3U128 |
別名 | FP7019, HTS, Drapc1, HHS, Apcdd1, B7323, HYPT1 |
Construction | Leu27-Thr492 |
Protein Purity | > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC |
分子量 | 54.4 kDa (predicted). Due to glycosylation, the protein migrates to 65-68 kDa based on Tris-Bis PAGE result. |
Endotoxin | < 1 EU/μg by the LAL method. |
Formulation | Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS (pH 7.4). Typically, 8% trehalose is incorporated as a protective agent before lyophilization. |
Reconstitution | Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing. |
Stability & Storage |
It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted protein solutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
Shipping |
In general, Lyophilized powders are shipping with blue ice. |
Research Background | Adenomatosis polyposis coli downregulated 1 (APCDD1), a negative regulator of Wnt signaling, was examined to understand detailed mechanisms underlying Wnt signaling tooth development. In situ hybridization showed that Apcdd1 was expressed in the condensed mesenchyme at the bud stage, and in the inner enamel epithelium (IEE), including enamel knot (EK) at the cap stage. APCDD1 modulates the gene expression of Wnt- and EK-related signaling molecules at the cap stage of tooth development, and is involved in tooth cusp patterning by modulating the epithelial rearrangement in the IEE. |
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Please read the User Guide of Recombinant Proteins for more specific information.
APCDD1 Protein, Mouse, Recombinant (His) B-7323 FP7019 HTS FP 7019 Drapc1 HHS HYPT-1 Apcdd1 B7323 B 7323 HYPT 1 FP-7019 HYPT1 recombinant recombinant-proteins proteins protein