| 説明 |
ATG5 is an E2 ubiquitin ligase which is necessary for autophagy. Its expression is a relatively late event in the apoptotic process, occurring downstream of caspase activity, dramatically highly expressed in apoptotic cells. It is activated by ATG7, conjugates to ATG12 and associates with isolation membrane to form cup-shaped isolation membrane and autophagosome. The conjugate complex detaches from the membrane immediately before or after autophagosome formation is completed. ATG5 plays an important role in the apoptotic process, possibly within the modified cytoskeleton. |
| Species |
Human
|
| Expression Host |
E. coli
|
| Tag |
None
|
| Accession Number |
Q9H1Y0
|
| 別名 |
ASP, APG5L, Apoptosis-specific protein, Autophagy protein 5, APG5-like, ATG5
|
| Amino Acid |
Met1-Asp275
|
| Construction |
Recombinant Human Autophagy Related 5 Homolog is produced by our E.coli expression system and the target gene encoding Met1-Asp275 is expressed.
|
| Protein Purity |
Greater than 90% as determined by reducing SDS-PAGE. (QC verified)
|
| 分子量 |
33 KDa, reducing conditions
|
| Endotoxin |
Less than 0.1 ng/µg (1 EU/µg) as determined by LAL test.
|
| Formulation |
Supplied as a 0.2 μm filtered solution of 20mM Tris-HCl, 0.2M NaCl, 1mM DTT, 40% Glycerol, pH 8.0.
|
| Stability & Storage |
Store at ≤-70°C, stable for 6 months after receipt.
Store at ≤-70°C, stable for 3 months under sterile conditions after opening.
Please minimize freeze-thaw cycles.
|
| Shipping |
The product is shipped on dry ice/polar packs.
Upon receipt, store it immediately at the temperature listed below.
|
| Research Background |
ATG5 is an E2 ubiquitin ligase which is necessary for autophagy. Its expression is a relatively late event in the apoptotic process, occurring downstream of caspase activity, dramatically highly expressed in apoptotic cells. It is activated by ATG7, conjugates to ATG12 and associates with isolation membrane to form cup-shaped isolation membrane and autophagosome. The conjugate complex detaches from the membrane immediately before or after autophagosome formation is completed. ATG5 plays an important role in the apoptotic process, possibly within the modified cytoskeleton.
|
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