B7-H3 Protein, Human, Recombinant (aa 29-245, His)

B7-H3, a member of the B7 family of immunomodulatory molecules, is overexpressed in a wide range of solid cancers.B7-H3 binds to activated T cells via an as yet unidentified receptor. In assays using sub-optimal amount so anti-CD3 stimulation, 2Ig‑B7‑H3 enhances T cell proliferation, T cell interferon-gamma (IFN-gamma) production, and cytotoxic T cells induction. B7-H3 Protein, Human, Recombinant (aa 29-245, His) is expressed in HEK293 mammalian cells with C-His tag. The predicted molecular weight is 24.7 kDa and the accession number is Q5ZPR3-2.

B7-H3 Protein, Human, Recombinant (aa 29-245, His)

パッケージサイズ	在庫状況	単価(税別)
100 μg	約5 days	￥ 85,500
500 μg	約5 days	￥ 342,000
1 mg	約5 days	￥ 569,500

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説明

Species	Human
Expression Host	HEK293 Cells
Tag	C-His
Accession Number	Q5ZPR3-2
別名	PRO352, B7H3, UNQ309, PSEC0249, CD276, B7-H3, B7 homolog 3
Construction	Leu29-Pro245
Protein Purity	> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
分子量	24.7 kDa (predicted). Due to glycosylation, the protein migrates to 40-50 kDa based on Tris-Bis PAGE result.

Endotoxin	< 1 EU/μg by the LAL method.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS (pH 7.4). Typically, 8% trehalose is incorporated as a protective agent before lyophilization.
Reconstitution	Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.
Stability & Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted proteinsolutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
Shipping	In general, Lyophilized powders are shipping with blue ice.
Research Background	B7-H3, a member of the B7 family of immunomodulatory molecules, is overexpressed in a wide range of solid cancers.B7-H3 binds to activated T cells via an as yet unidentified receptor. In assays using sub-optimal amount so anti-CD3 stimulation, 2Ig‑B7‑H3 enhances T cell proliferation, T cell interferon-gamma (IFN-gamma) production, and cytotoxic T cells induction.

参考文献

1. Fodstad O, et al. The Immunoregulatory Protein Human B7H3 is a Tumor-Associated Antigen that Regulates Tumor Cell Migration and Invasion[J]. Current Cancer Drug Targets, 2008, 8(5)

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B7-H3 Protein, Human, Recombinant (aa 29-245, His)

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