B7-H3 Protein, Human, Recombinant (His & Avi), Biotinylated is expressed in HEK293 mammalian cells with His and Avi tag. The predicted molecular weight is 49.8 kDa and the accession number is Q5ZPR3.
パッケージサイズ | 在庫状況 | 単価(税別) |
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20 μg | 在庫あり | ¥ 46,500 |
100 μg | 約5 days | ¥ 109,500 |
200 μg | 約5 days | ¥ 186,500 |
500 μg | 約5 days | ¥ 376,500 |
生物学的情報 |
Immobilized Anti-B7-H3 Antibody, IgG1 Kappa at 2 μg/mL (100 μL/well) can bind Recombinant Human B7-H3 Protein (His & AVI Tag), Biotinylated , the EC50 is 4.3-13 ng/mL.
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説明 | B7-H3 Protein, Human, Recombinant (His & Avi), Biotinylated is expressed in HEK293 mammalian cells with His and Avi tag. The predicted molecular weight is 49.8 kDa and the accession number is Q5ZPR3. |
Species | Human |
Expression Host | HEK293 Cells |
Tag | His, Avi |
Accession Number | Q5ZPR3 |
別名 | 4Ig-B7-H3, B7-H3, B7H3, CD276 molecule, B7RP-2 |
Construction | The Human CD276 (Q5ZPR3) (Met1-Thr461) was expressed with a c-terminal polyhistidine tagged AVI tag at the C-terminus. The expressed protein was biotinylated in vivo by the Biotin-Protein ligase (BirA enzyme) which is co-expressed. |
Protein Purity |
> 95 % as determined by SDS-PAGE.
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分子量 | 49.8 kDa (predicted) |
Endotoxin | < 1.0 EU/μg of the protein as determined by the LAL method. |
Formulation | Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization. |
Reconstitution | A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information. |
Stability & Storage |
It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted proteinsolutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
Shipping |
In general, Lyophilized powders are shipping with blue ice. |
Research Background | B7-H3 is a member of the B7 family of immune regulatory ligands that is thought to attenuate peripheral immune responses through co-inhibition. It plays an important role in adaptive immune responses, and was shown to either promote or inhibit T-cell responses in various experimental systems. B7-H3 may play an important role in muscle-immune interactions, providing further evidence of the active role of muscle cells in local immunoregulatory processes. B7-H3 is a novel protein structurally related to the B7 family of ligands by the presence of a single set of immunoglobulin-V-like and immunoglobulin-C-like (VC) domains. Previous studies have correlated its overexpression with poor prognosis and decreased tumor-infiltrating lymphocytes in various carcinomas including uterine endometrioid carcinomas, and mounting evidence supports an immuno-inhibitory role in ovarian cancer prognosis. Recently, B7-H3 expression has been reported in several human cancers indicating an additional function of B7-H3 as a regulator of antitumor immunity.Cancer ImmunotherapyCo-inhibitory Immune Checkpoint TargetsImmune CheckpointImmune Checkpoint Detection: AntibodiesImmune Checkpoint Detection: ELISA AntibodiesImmune Checkpoint Detection: FCM AntibodiesImmune Checkpoint Detection: ICC AntibodiesImmune Checkpoint Detection: IP AntibodiesImmune Checkpoint Detection: WB AntibodiesImmune Checkpoint TargetsImmunotherapyTargeted Therapy |
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Please read the User Guide of Recombinant Proteins for more specific information.
B7-H3 Protein, Human, Recombinant (His & Avi), Biotinylated 4Ig-B7-H3 B7-H3 B7H3 CD276 molecule B7RP-2 recombinant recombinant-proteins proteins protein