BMP-4 Protein, Human, Recombinant (E399D) is expressed in E. coli expression system. The predicted molecular weight is 13.25 kDa and the accession number is P12644.
| 生物学的情報 |
Measured by its ability to induce alkaline phosphatase production by MC3T3-E1 mouse osteoblastic cells. The ED50 for this effect is typically 0.08-0.4ug/mL.
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| 説明 |
BMP-4 Protein, Human, Recombinant (E399D) is expressed in E. coli expression system. The predicted molecular weight is 13.25 kDa and the accession number is P12644. |
| Species |
Human
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| Expression Host |
E. coli
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| Tag |
Tag Free
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| Accession Number |
P12644
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| 別名 |
OFC11, BMP2B, bone morphogenetic protein 4, BMP2B1, ZYME, MCOPS6
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| Construction |
A DNA sequence encoding the human BMP4 (P12644) (Ser293-Arg408, E399D) was expressed.
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| Protein Purity |
≥ 95 % as determined by SDS-PAGE.
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| 分子量 |
13.25 kDa (predicted)
|
| Endotoxin |
< 1.0 EU per μg protein as determined by the LAL method.
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| Formulation |
Lyophilized from sterile 30% Acetonitrile, 1/1000 TFA. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0. 01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
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| Reconstitution |
A hardcopy of datasheet with reconstitution instructions is sent along with the products. Please refer to it for detailed information.
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| Stability & Storage |
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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| Shipping |
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
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| Research Background |
BMP4 (bone morphogenetic protein-4)-directed differentiation of human embryonic stem cells (ESCd). Autophagy is a conserved catabolic process with complicated roles in tumor development. Bone morphogenetic protein 4 (BMP4), a member of the transforming growth factor (TGF-β) family of regulatory proteins, plays a crucial role in human malignancies. BMP4 treatment promoted HCC cells proliferation and induced autophagy. Mechanistic study revealed that the induction of autophagy by BMP4 was mediated through activating the JNK1/Bcl2 pathway. And the JNK1 inhibitor and knockdown of JNK1 could attenuate autophagy induced by BMP4 and eliminated BMP4-promoted HCC cells growth. BMP4 promoted HCC proliferation by autophagy activation through JNK1/Bcl-2 signaling.
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