C-Reactive Protein Protein, Human, Recombinant is expressed in HEK293 mammalian cells. The predicted molecular weight is 23 kDa and the accession number is P02741-1.
| 生物学的情報 |
Testing in progress
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| 説明 |
C-Reactive Protein Protein, Human, Recombinant is expressed in HEK293 mammalian cells. The predicted molecular weight is 23 kDa and the accession number is P02741-1. |
| Species |
Human
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| Expression Host |
HEK293 Cells
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| Tag |
Tag Free
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| Accession Number |
P02741-1
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| 別名 |
C-Reactive Protein, C-reactive protein, pentraxin-related, PTX1
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| Construction |
The Human CRP (P02741-1) (Met1-Pro224) was expressed and purified.
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| Protein Purity |
≥ 90 % as determined by SDS-PAGE ≥ 95 % as determined by SEC-HPLC.
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| 分子量 |
23 kDa (predicted)
|
| Endotoxin |
< 1.0 EU/μg of the protein as determined by the LAL method.
|
| Formulation |
Lyophilized from a solution filtered through a 0.22 μm filter, containing 20 mM Tris, 0.2M NaCl, 5 mM CaCl2, pH 8.0.Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
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| Reconstitution |
A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
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| Stability & Storage |
It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted proteinsolutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
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| Shipping |
In general, Lyophilized powders are shipping with blue ice.
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| Research Background |
C-reactive protein (CRP) is synthesized by the liver in response to factors released by fat cells. It is a member of the pentraxin family of proteins. The levels of CRP rise in response to inflammation. Human C-reactive protein (CRP) is the classical acute phase reactant, the circulating concentration of which rises rapidly and extensively in a cytokine-mediated response to tissue injury, infection and inflammation. Serum CRP values are routinely measured, empirically, to detect and monitor many human diseases. However, CRP is likely to have important host defence, scavenging and metabolic functions through its capacity for calcium-dependent binding to exogenous and autologous molecules containing phosphocholine (PC) and then activating the classical complement pathway. CRP may also have pathogenic effects and the recent discovery of a prognostic association between increased CRP production and coronary atherothrombotic events is of particular interest.
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参考文献
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