CA5A Protein, Human, Recombinant (His)

Carbonic anhydrase 5A, mitochondrial, also known as Carbonate dehydratase VA, Carbonic anhydrase VA, CA-VA and CA5A, is a member of thealpha-carbonic anhydrase family. Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes first discovered in 1933 that catalyze the reversible hydration of carbon dioxide. CAs participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. CA5A / CA-VA is activated by histamine, L-adrenaline, L- and D-histidine, and L- and D-phenylalanine. It is inhibited by coumarins, sulfonamide derivatives such as acetazolamide and Foscarnet (phosphonoformate trisodium salt).

CA5A Protein, Human, Recombinant (His)

パッケージサイズ	在庫状況	単価(税別)
50 μg	約5 days	￥ 114,500

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説明

Species	Human
Expression Host	E. coli
Tag	C-His
Accession Number	P35218
別名	CA5, GS1-21A4.1, CA5AD, carbonic anhydrase VA, mitochondrial, CA5D, CAV, Carbonic Anhydrase VA, CAVA
Construction	The mature form of Human CA5A (NP_001730.1) (Ala 40-Ser 305) was fused with an Met at N-terminus and a polyhistide tag at the C-terminus.
Protein Purity	> 96 % as determined by SDS-PAGE
分子量	31.6 kDa (predicted)

Endotoxin	Please contact us for more information.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing 50 mM NaAc, 50 mM NaCl, 0.05% Brij 35, pH 5. 0.Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
Reconstitution	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
Stability & Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted proteinsolutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
Shipping	In general, Lyophilized powders are shipping with blue ice.
Research Background	Carbonic anhydrase 5A, mitochondrial, also known as Carbonate dehydratase VA, Carbonic anhydrase VA, CA-VA and CA5A, is a member of thealpha-carbonic anhydrase family. Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes first discovered in 1933 that catalyze the reversible hydration of carbon dioxide. CAs participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. CA5A / CA-VA is activated by histamine, L-adrenaline, L- and D-histidine, and L- and D-phenylalanine. It is inhibited by coumarins, sulfonamide derivatives such as acetazolamide and Foscarnet (phosphonoformate trisodium salt).

参考文献

1. Strausberg, R.L. et al., 2002, Proc. Natl. Acad. Sci. USA 99:16899 - 903. 2. Liao, S.Y. et al., 2003, J. Med. Genet. 40:257 - 262. 3. Temperini C.et al., 2006, Chemistry 12: 7057-66. 4. Temperini C.et al., 2006, J. Med. Chem. 49: 3019-27. 5. Supuran, C. T. et al., 2008, Curr Pharm Des. 14 (7): 601-602. 6. Elleuche, S. et al., 2009, Curr Genet. 55 (2): 211-222.

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CA5A Protein, Human, Recombinant (His)

参考文献

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