Carboxypeptidase A2 Protein, Human, Recombinant (His)

Carboxypeptidase A2 Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 46 kDa and the accession number is P15085.

Carboxypeptidase A2 Protein, Human, Recombinant (His)

パッケージサイズ	在庫状況	単価(税別)
50 μg	約5 days	￥ 138,000

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説明	Carboxypeptidase A2 Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 46 kDa and the accession number is P15085.

Species	Human
Expression Host	HEK293 Cells
Tag	C-His
Accession Number	P15085
別名	carboxypeptidase A2, CPA2
Construction	pre pro form of Human CPA2 (NP_001859.1) (Met 1-Tyr 417)
Protein Purity	> 90 % as determined by SDS-PAGE
分子量	46 kDa (predicted)

Endotoxin	< 1.0 EU/μg of the protein as determined by the LAL method.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing 25 mM Tris, 0.15 mM NaCl, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
Reconstitution	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
Stability & Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted proteinsolutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
Shipping	In general, Lyophilized powders are shipping with blue ice.
Research Background	Carboxypeptidase A2 ( CPA2 ) is a secreted pancreatic procarboxy -peptidase, and cleaves the C-terminal amide or ester bond of peptides that have a free C-terminal carboxyl group. The hydrolytic action of CPA2 was identified with a preference towards long substrates with aromatic amino acids in their C-terminal end, particularly tryptophan. CPA2 comprises a signal peptide, a pro region and a mature chain, and can be activated after cleavage of the pro peptide. Three different forms of human pancreatic procarboxypeptidase A have been isolated, and the A1 and A2 forms are always secreted as monomeric proteins with different biochemical properties.

参考文献

1. Catasus, L. et al., 1995. J. Biol. Chem. 270: 6651-6657. 2. Aloy, P. et al., 1998, Biol. Chem. 379: 149-155. 3. Laethem, RM. et al., 1996, Arch. Biochem. Biophys.332: 8-18.

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Carboxypeptidase A2 Protein, Human, Recombinant (His)

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