Carboxypeptidase B1 Protein, Human, Recombinant (His)

Carboxypeptidase B1 Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 47 kDa and the accession number is P15086.

Carboxypeptidase B1 Protein, Human, Recombinant (His)

パッケージサイズ	在庫状況	単価(税別)
50 μg	約5 days	￥ 138,000

バルクのお問い合わせ

説明	Carboxypeptidase B1 Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 47 kDa and the accession number is P15086.

Species	Human
Expression Host	HEK293 Cells
Tag	C-His
Accession Number	P15086
別名	PASP, PCPB, carboxypeptidase B1 (tissue), CPB
Construction	Human CPB1 (NP_001862.2) (Met 1-Tyr 417)
Protein Purity	> 98 % as determined by SDS-PAGE
分子量	47 kDa (predicted)

Endotoxin	< 1.0 EU/μg of the protein as determined by the LAL method.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing 25 mM MES, 0.1 M NaCl, pH 6. 5. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
Reconstitution	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
Stability & Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted protein solutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
Shipping	In general, Lyophilized powders are shipping with blue ice.
Research Background	Carboxypeptidase B1, also well known as pancreatic procarboxypeptidase B (PCPB), is a highly pancreas -specific protein (PASP), and has been identified previously as a serum marker for acute pancreatitis and pancreatic graft rejection. As the prototype for those human exopeptidases that cleave off basic C-terminal residues, CPB1 specifically cleaves the C-terminal Arg and Lys residues with a preference for Arg. The B1 and B2 forms of procarboxypeptidase B differ from each other mainly in isoelectric point.The deduced amino acid sequence of PCPB predicts a 416-amino acid preproenzyme consisting of a 15-aa signal peptide, a 95-aa activation peptide and a 37-aa mature chain. The secreted PCPB zymogen is converted to enzymatically active CPB1 by limited proteolysis by trypsin.

参考文献

1. Yamamoto, K.K. et al., 1992, J. Biol. Chem. 267: 2575-2581. 2. Pezzilli, R. et al., 1994, Digestion. 55: 73-77. 3. Barbosa Pereira, P.J. et al., 2002, J. Mol. Biol. 321: 537-547.

Calculator

Reconstitution Calculator

Recombinant Proteins Dilute Calculator

Specific Activity Calculator

bottom

Carboxypeptidase B1 Protein, Human, Recombinant (His)

参考文献

Calculator

技術サポート

Keywords