CCK4/PTK7 Protein, Cynomolgus, Rhesus, Recombinant (His)

CCK4/PTK7 Protein, Cynomolgus, Rhesus, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 76.1 kDa and the accession number is XP_005552998.1&NP_001248508.1.

CCK4/PTK7 Protein, Cynomolgus, Rhesus, Recombinant (His)

パッケージサイズ	在庫状況	単価(税別)
100 μg	約5 days	￥ 161,000
1 mg	約5 days	￥ 1,049,000

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説明	CCK4/PTK7 Protein, Cynomolgus, Rhesus, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 76.1 kDa and the accession number is XP_005552998.1&NP_001248508.1.

Species	Cynomolgus,Rhesus
Expression Host	HEK293 Cells
Tag	C-His
Accession Number	XP_005552998.1&NP_001248508.1
Construction	The cynomolgus/rhesus PTK7 (XP_005552998.1/NP_001248508.1) (Met1-Thr704) was expressed with a polyhistidine tag at the C-terminus.
Protein Purity	≥ 95 % as determined by SDS-PAGE. ≥ 90 % as determined by SEC-HPLC.
分子量	76.1 kDa (predicted)

Endotoxin	< 1.0 EU/μg of the protein as determined by the LAL method.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
Reconstitution	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
Stability & Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted proteinsolutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
Shipping	In general, Lyophilized powders are shipping with blue ice.
Research Background	Protein tyrosine kinase 7 (PTK7) is a highly conserved but catalytically inactive receptor tyrosine kinase in the Wnt signaling pathway. PTK7 regulates various processes in embryonic development and tissue homeostasis. On a cellular level PTK7 affects the establishment of cell polarity, the regulation of cell movement and migration as well as cell invasion. The PTK7 receptor interacts with ligands, co-receptors, and intracellular transducers of Wnt signaling pathways, pointing to a function in the fine-tuning of the Wnt signaling network. PTK7 is a critical regulator of canonical and non-canonical Wnt-signaling during embryonic development and cancer cell formation. Disrupting PTK7 activity perturbs vertebrate nervous system development, and also promotes human cancer formation. PTK7 plays an important role in human cancers, including triple-negative breast cancer (TNBC), ovarian cancer, and non-small cell lung cancer (NSCLC).

参考文献

1. Lichtig H,et al.(2019) Ptk7 proteolytic fragment proteins function during early xenopus development. Dev Biol 453 (1): 48-55. 2. Damelin M,et al.(2017) A ptk7-targeted antibody-drug conjugate reduces tumor-initiating cells and induces sustained tumor regressions. Sci Transl Med 9 (372): eaag2611. 3. Berger H,et al.(2017) Ptk7 faces the wnt in development and disease. Front Cell Dev Biol 5 31.

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CCK4/PTK7 Protein, Cynomolgus, Rhesus, Recombinant (His)

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