Aminopeptidase N/CD13 Protein, Human, Recombinant (I603M, His)

Aminopeptidase N/CD13 Protein, Human, Recombinant (I603M, His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 104.2 kDa and the accession number is A0A024RC61.

Aminopeptidase N/CD13 Protein, Human, Recombinant (I603M, His)

パッケージサイズ	在庫状況	単価(税別)
50 μg	在庫あり	￥ 88,500
100 μg	約5 days	￥ 151,500
200 μg	約5 days	￥ 257,000
500 μg	約5 days	￥ 521,000

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生物学的情報	Measured by its ability to cleave the fluorogenic peptide substrate, Ala-7-amido-4-methylcoumarin (Ala-AMC). The specific activity is >2,500 pmoles/min/μg.
説明	Aminopeptidase N/CD13 Protein, Human, Recombinant (I603M, His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 104.2 kDa and the accession number is A0A024RC61.

Species	Human
Expression Host	HEK293
Tag	His
Accession Number	A0A024RC61
別名	alanyl (membrane) aminopeptidase, LAP1, APN, CD13, P150, PEPN, GP150
Construction	A DNA sequence encoding the metalloprotease domain (Lys 69-Lys 967，I603M) of the mature form of human ANPEP (NP_001141.2) was expressed with N-terminal signal peptide and C-terminal polyhistidine tag.
Protein Purity	> 97 % as determined by SDS-PAGE
分子量	104.2 kDa (predicted)

Endotoxin	< 1.0 EU per μg of the protein as determined by the LAL method.
Formulation	Lyophilized from sterile PBS, pH 7.3. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0. 01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
Reconstitution	A hardcopy of datasheet with reconstitution instructions is sent along with the products. Please refer to it for detailed information.
Stability & Storage	Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Shipping	In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Research Background	Aminopeptidase N (ANPEP or APN), also known as CD13, is a cell-surface metalloprotease located in the small-intestinal and renal microvillar membrane, as well as other plasma membranes. It belongs to the peptidase M1 family. CD13 plays a role in the final digestion of peptides generated from hydrolysis of proteins by gastric and pancreatic proteases and is involved in the metabolism of regulatory peptides by diverse cell types. CD13/APN is a potent regulator of angiogenesis which is essential for tumor invasion and metastasis, and its transcription in activated endothelial cells is induced by angiogenic growth factors via the RAS/MAPK pathway. In addition, this enzyme has been shown to participate in antigen processing and presentation, and accordingly, defects in this gene appear to be a cause of various types of leukemia or lymphoma and carcinomas.

参考文献

1. Pasqualini R,et al.(2000) Aminopeptidase N is a receptor for tumor-homing peptides and a target for inhibiting angiogenesis.Cancer Res. 60(3): 722-7. 2. Mabjeesh SJ,et al.(2005) Aminopeptidase N gene expression and abundance in caprine mammary gland is influenced by circulating plasma peptide. J Dairy Sci. 88(6): 2055-64. 3. Dunkel B,et al.(2009) Neutrophil and platelet activation in equine recurrent airway obstruction is associated with increased neutrophil CD13 expression, but not platelet CD41/61 and CD62P or neutrophil-platelet aggregate formation. Vet Immunol Immunopathol. 131(1-2): 25-32.

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Aminopeptidase N/CD13 Protein, Human, Recombinant (I603M, His)

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