CD146/MCAM Protein, Mouse, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 61.3 kDa and the accession number is Q8R2Y2-1.
説明 | CD146/MCAM Protein, Mouse, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 61.3 kDa and the accession number is Q8R2Y2-1. |
Species | Mouse |
Expression Host | HEK293 Cells |
Tag | His |
Accession Number | Q8R2Y2-1 |
別名 | CD146, melanoma cell adhesion molecule, CD149, s-gicerin, AV025631, Muc18, 1-gicerin, s-endo |
Construction | The Mouse MCAM (Q8R2Y2-1) extracellular domain (Met 1-Val 563) was expressed, fused with a C-terminal polyhistidine tag. |
Protein Purity | > 95 % as determined by SDS-PAGE |
分子量 | 61.3 kDa (predicted) |
Endotoxin | < 1.0 EU/μg of the protein as determined by the LAL method. |
Formulation | Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization. |
Reconstitution | A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information. |
Stability & Storage |
It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted proteinsolutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
Shipping |
In general, Lyophilized powders are shipping with blue ice. |
Research Background | The CD146 antigen, also known as melanoma cell adhesion molecule (MCAM) and MUC18, is an integral membrane glycoprotein belonging to the immunoglobulin superfamily. CD146 contains the characteristic immunoglobulin-like domains (V-V-C2-C2-C2), a transmembrane region, and a short cytoplasmic tail. The CD146 expression is detected in endothelial cells in vascular tissue throughout the body and plays a role in cell adhesion, as well as in cohesion of the endothelial monolayer at intercellular junctions in vascular tissue. As a Ca2+-independent cell adhesion molecule involved in heterophilic cell to cell interactions and a surface receptor, CD146 triggers tyrosine phosphorylation of FYN and PTK2 and subsequently induced signal transduction, proteolysis, or immune recognition. This protein is also expressed predominantly on metastatic lesions and advanced primary tumors, and thus has been suggested to play an important role in tumor progression and the development of metastasis in certain human carcinomas. |
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Please read the User Guide of Recombinant Proteins for more specific information.
CD146/MCAM Protein, Mouse, Recombinant (His) CD 146 CD146 melanoma cell adhesion molecule CD149 s-gicerin CD-149 AV025631 Muc18 AV 025631 1-gicerin CD 149 s-endo AV-025631 CD-146 recombinant recombinant-proteins proteins protein