CD16a Protein, Cynomolgus, Recombinant (His), Biotinylated is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 23.1 kDa and the accession number is Q8SPW2.1.
パッケージサイズ | 在庫状況 | 単価(税別) |
---|---|---|
20 μg | 在庫あり | ¥ 45,500 |
100 μg | 約5 days | ¥ 107,500 |
200 μg | 約5 days | ¥ 183,000 |
500 μg | 約5 days | ¥ 369,500 |
生物学的情報 | Testing in progress |
説明 | CD16a Protein, Cynomolgus, Recombinant (His), Biotinylated is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 23.1 kDa and the accession number is Q8SPW2.1. |
Species | Cynomolgus |
Expression Host | HEK293 Cells |
Tag | C-His |
Accession Number | Q8SPW2.1 |
別名 | Fc fragment of IgG, low affinity IIIa, receptor (CD16a) |
Construction | The cynomolgus FCGR3 (Q8SPW2.1) (Met1-Gly206) was expressed with a polyhistidine tag at the C-terminus. The purified protein was biotinylated in vitro. |
Protein Purity |
> 90 % as determined by SDS-PAGE.
|
分子量 | 23.1 kDa (predicted) |
Endotoxin | < 1.0 EU/μg of the protein as determined by the LAL method. |
Formulation | Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization. |
Reconstitution | A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information. |
Stability & Storage |
It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted proteinsolutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
Shipping |
In general, Lyophilized powders are shipping with blue ice. |
Research Background | The Fc receptor with low affinity for IgG (FCGR3, or CD16) is encoded by 2 nearly identical genes, FCGR3A and FCGR3B, resulting in tissue-specific expression of alternative membrane-anchored isoforms. FCGR3A, it is also known as CD16a, encodes a transmembrane protein expressed on activated monocytes/macrophages, natural killer (NK) cells, and a subset of T cells. CD16a / FCGR3A is a receptor expressed on NK cells that facilitates antibody dependent cellular cytotoxicity (ADCC) by binding to the Fc portion of various antibodies. CD16a / FCGR3A also has a broader function. CD16a / FCGR3A is directly involved in the lysis of some virus-infected cells and tumor cells by NK cells, independent of antibody binding. Cross-linking of CD16a / FCGR3A on NK cells resulted in increased intracellular Ca2+ levels and a cascade of biochemical events similar to those activated by the T cell receptor. CD16a / FCGR3A on human NK cells is a lysis receptor that mediates the direct killing of some virus infected and tumor cells, independent of antibody ligation. |
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CD16a Protein, Cynomolgus, Recombinant (His), Biotinylated Fc fragment of IgG, low affinity IIIa, receptor Fc fragment of IgG, low affinity IIIa, receptor (CD16a) CD16a recombinant recombinant-proteins proteins protein