CD16a Protein, Human, Recombinant (176 Phe, His) is expressed in CHO mammalian cells with His tag. The predicted molecular weight is 23.3 kDa and the accession number is P08637-1.
説明 | CD16a Protein, Human, Recombinant (176 Phe, His) is expressed in CHO mammalian cells with His tag. The predicted molecular weight is 23.3 kDa and the accession number is P08637-1. |
Species | Human |
Expression Host | CHO Cells |
Tag | C-His |
Accession Number | P08637-1 |
別名 | IGFR3, CD16, FCR-10, FCRIIIA, CD16A, Fc γ RIIIa, Fc fragment of IgG, low affinity IIIa, receptor (CD16a), IMD20, FCGR3, FCRIII, Fc gamma RIIIa, FCG3, FCGRIII |
Construction | extracellular domain (Met 1-Gln 208) of Human CD16a (P08637-1) precursor |
Protein Purity | ≥ 95 % as determined by SDS-PAGE. ≥ 90 % as determined by SEC-HPLC. |
分子量 | 23.3 kDa (predicted) |
Endotoxin | < 1.0 EU/μg of the protein as determined by the LAL method. |
Formulation | Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization. |
Reconstitution | A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information. |
Stability & Storage |
It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted proteinsolutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
Shipping |
In general, Lyophilized powders are shipping with blue ice. |
Research Background | The Fc receptor with low affinity for IgG (FCGR3, or CD16) is encoded by 2 nearly identical genes, FCGR3A and FCGR3B, resulting in tissue-specific expression of alternative membrane-anchored isoforms. FCGR3A, it is also known as CD16a, encodes a transmembrane protein expressed on activated monocytes/macrophages, natural killer (NK) cells, and a subset of T cells. CD16a / FCGR3A is a receptor expressed on NK cells that facilitates antibody dependent cellular cytotoxicity (ADCC) by binding to the Fc portion of various antibodies. CD16a / FCGR3A also has a broader function. CD16a / FCGR3A is directly involved in the lysis of some virus-infected cells and tumor cells by NK cells, independent of antibody binding. Cross-linking of CD16a / FCGR3A on NK cells resulted in increased intracellular Ca2+ levels and a cascade of biochemical events similar to those activated by the T cell receptor. CD16a / FCGR3A on human NK cells is a lysis receptor that mediates the direct killing of some virus infected and tumor cells, independent of antibody ligation. |
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Please read the User Guide of Recombinant Proteins for more specific information.
CD16a Protein, Human, Recombinant (176 Phe, His) IMD 20 IGFR3 FCG 3 CD16 Fc fragment of IgG, low affinity IIIa, receptor FCR-10 FCGR-3 FCRIIIA FCGR 3 FCR 10 CD-16 CD16A Fc γ RIIIa IMD-20 Fc fragment of IgG, low affinity IIIa, receptor (CD16a) CD 16 Fc gamma RIIIa IMD20 FCGR3 FCRIII IGFR-3 IGFR 3 FCG-3 Fc gamma RIIIa FCR10 FCG3 FCGRIII CD16a recombinant recombinant-proteins proteins protein