CD1D Protein, Mouse, Recombinant (His & hFc)

CD1D Protein, Mouse, Recombinant (His & hFc) is expressed in HEK293 mammalian cells with His and hFc tag. The predicted molecular weight is 60.4 kDa and the accession number is A0A0R4J090.

CD1D Protein, Mouse, Recombinant (His & hFc)

パッケージサイズ	在庫状況	単価(税別)
100 μg	約5 days	￥ 138,000

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説明	CD1D Protein, Mouse, Recombinant (His & hFc) is expressed in HEK293 mammalian cells with His and hFc tag. The predicted molecular weight is 60.4 kDa and the accession number is A0A0R4J090.

Species	Mouse
Expression Host	HEK293 Cells
Tag	His, hFc
Accession Number	A0A0R4J090
別名	CD1.1, Cd1a, Cd1d, CD1d molecule, AI747460, Ly-38
Construction	The extracellular domain of Mouse CD1D (NP_031665.2) (Met 1-Gly 305) was fused with the C-terminal polyhistidine-tagged Fc region of Human IgG1 at the C-terminus.
Protein Purity	> 80 % as determined by SDS-PAGE
分子量	60.4 kDa (predicted)

Endotoxin	< 1.0 EU/μg of the protein as determined by the LAL method.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing 20 mM Tris, 150 mM NaCl, 10% glycerol, pH 8.0.Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
Reconstitution	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
Stability & Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted proteinsolutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
Shipping	In general, Lyophilized powders are shipping with blue ice.
Research Background	The cluster of differentiation (CD) system is commonly used as cell markers in Immunophenotyping. Different kinds of cells in the immune system can be identified through the surface CD molecules associating with the immune function of the cell. There are more than 320 CD unique clusters and subclusters have been identified. Some of the CD molecules serve as receptors or ligands important to the cell through initiating a signal cascade which then alter the behavior of the cell. Some CD proteins do not take part in cell signal process but have other functions such as cell adhesion. Cluster of differentiation 1 (CD1) is a member of the CD system. It's a family of glycoproteins expressed on the surface of various human antigen-presenting cells which are implicated in the presentation of lipid antigens to T-cells. Due to the different lipid anchoring, the CD1 family is classified into two groups: group1 (CD1a-c) and group2 (CD1d). CD1d with lipid antigens activate NK T-cells which rapidly produce Th1 and Th2 cytokines after been activated.

参考文献

1. Zola H,et al.(2007) CD molecules 2006-human cell differentiation molecules. J Immunol Methods. 318 (1-2): 1-5. 2. Ho IC,et al.(2009) GATA3 and the T-cell lineage: essential functions before and after T-helper-2-cell differentiation. Nat Rev Immunol. 9 (2): 125-35. 3. Matesanz-Isabel J,et al.(2011) New B-cell CD molecules. Immunology Letters.134 (2): 104-12. 4. Joyce S.(2001) CD1d and natural T cells: how their properties jump-start the immune system. Cell Mol Life Sci. 58 (3): 442-69.

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CD1D Protein, Mouse, Recombinant (His & hFc)

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