Siglec-2/CD22 Protein, Human, Recombinant (aa 176-687, His), Biotinylated

Siglec-2/CD22 Protein, Human, Recombinant (aa 176-687, His), Biotinylated is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 58.5 kDa and the accession number is Q32M46.

Siglec-2/CD22 Protein, Human, Recombinant (aa 176-687, His), Biotinylated

パッケージサイズ	在庫状況	単価(税別)
20 μg	約5 days	￥ 70,500
100 μg	約5 days	￥ 167,000

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説明	Siglec-2/CD22 Protein, Human, Recombinant (aa 176-687, His), Biotinylated is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 58.5 kDa and the accession number is Q32M46.

Species	Human
Expression Host	HEK293 Cells
Tag	C-His
Accession Number	Q32M46
別名	CD22 molecule, SIGLEC-2, SIGLEC2
Construction	The Human CD22 (Q32M46) (Trp176-Arg687) was fused with a polyhistidine tag at the C-terminus. The purified protein was biotinylated in vitro.
Protein Purity	> 90 % as determined by SDS-PAGE
分子量	58.5 kDa (predicted)

Endotoxin	< 1.0 EU/μg of the protein as determined by the LAL method.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
Reconstitution	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
Stability & Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted proteinsolutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
Shipping	In general, Lyophilized powders are shipping with blue ice.
Research Background	CD22 is a member of the immunoglobulin superfamily, SIGLEC family of lectins. It is first expressed in the cytoplasm of pro-B and pre-B cells, and on the surface as B cells mature to become IgD+. CD22 serves as an adhesion receptor for sialic acid-bearing ligands expressed on erythrocytes and all leukocyte classes. In addition to its potential role as a mediator of intercellular interactions, signal transduction through CD22 can activate B cells and modulate antigen receptor signaling in vitro. The phenotype of CD22-deficient mice suggests that CD22 is primarily involved in the generation of mature B cells within the bone marrow, blood, and marginal zones of lymphoid tissues. CD22 recruits the tyrosine phosphatase Src homology 2 domain-containing phosphatase 1 (SHP-1) to immunoreceptor tyrosine-based inhibitory motifs (ITIMs) and inhibits B-cell receptor (BCR)-induced Ca2+ signaling on normal B cells. CD22 interacts specifically with ligands carrying alpha2-6-linked sialic acids. As an inhibitory coreceptor of the B-cell receptor (BCR), CD22 plays a critical role in establishing signalling thresholds for B-cell activation. Like other coreceptors, the ability of CD22 to modulate B-cell signalling is critically dependent upon its proximity to the BCR, and this in turn is governed by the binding of its extracellular domain to alpha2,6-linked sialic acid ligands. However, genetic studies in mice reveal that some CD22 functions are regulated by ligand binding, whereas other functions are ligand-independent and may only require expression of an intact CD22 cytoplasmic domain at the B-cell surface. CD19 regulates CD22 phosphorylation by augmenting Lyn kinase activity, while CD22 inhibits CD19 phosphorylation via SHP-1.Cancer ImmunotherapyImmune CheckpointImmunotherapyTargeted Therapy

参考文献

1. Tedder TF,et al.(1997) CD22, a B lymphocyte-specific adhesion molecule that regulates antigen receptor signaling. Annu Rev Immunol. 15: 481-504. 2. Tedder TF,et al.(2005) CD22: a multifunctional receptor that regulates B lymphocyte survival and signal transduction. Adv Immunol. 88: 1-50. 3. Fujimoto M,et al.(2007) B cell signaling and autoimmune diseases: CD19/CD22 loop as a B cell signaling device to regulate the balance of autoimmunity. J Dermatol Sci. 46(1): 1-9. 4. Walker JA,et al.(2008) CD22: an inhibitory enigma. Immunology. 123(3): 314-25. 5. Nitschke L. (2009) CD22 and Siglec-G: B-cell inhibitory receptors with distinct functions. Immunol Rev. 230(1): 128-43.

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Siglec-2/CD22 Protein, Human, Recombinant (aa 176-687, His), Biotinylated

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