CD28 Protein, Human/Cynomolgus, Recombinant (His) is expressed in HEK293 mammalian cells with N-6xHis tag. The predicted molecular weight is 30-40 KDa and the accession number is P10747.
| 生物学的情報 |
1. Loaded Anti-Human CD28 mAb-Fc on Protein A Biosensor, can bind Human/Cynomolgus CD28-His with an affinity constant of 0.77 nM as determined in BLI assay. (Regularly tested)
2. Immobilized Human/Cynomolgus CD28-His at 2μg/ml (100 μl/well) can bind Anti-CD28 mAb The ED50 of Anti-CD28 mAb is 15.3 ng/ml. (Regularly tested)
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| 説明 |
CD28 Protein, Human/Cynomolgus, Recombinant (His) is expressed in HEK293 mammalian cells with N-6xHis tag. The predicted molecular weight is 30-40 KDa and the accession number is P10747. |
| Species |
Human,Cynomolgus
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| Expression Host |
Human Cells
|
| Tag |
N-6His
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| Accession Number |
P10747
|
| 別名 |
CD28 antigen, CD28 molecule, T-cell-specific surface glycoprotein CD28, CD28, Tp44
|
| Amino Acid |
Asn19-Pro152
|
| Construction |
Recombinant Human T-cell-specific Surface Glycoprotein CD28 is produced by our Mammalian expression system and the target gene encoding Asn19-Pro152 is expressed with a 6His tag at the N-terminus.
|
| Protein Purity |
Greater than 95% as determined by reducing SDS-PAGE. (QC verified)
|
| 分子量 |
30-40 KDa, reducing conditions
|
| Endotoxin |
Less than 0.1 ng/µg (1 EU/µg) as determined by LAL test.
|
| Formulation |
Lyophilized from a 0.2 μm filtered solution of 20mM Tris-HCl, 150mM NaCl, pH 8.0.
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| Reconstitution |
Always centrifuge tubes before opening.Do not mix by vortex or pipetting.
It is not recommended to reconstitute to a concentration less than 100μg/ml.
Dissolve the lyophilized protein in distilled water.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
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| Stability & Storage |
Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt.
Reconstituted protein solution can be stored at 2-8°C for 2-7 days.
Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
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| Shipping |
The product is shipped at ambient temperature.
Upon receipt, store it immediately at the temperature listed below.
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| Research Background |
T-cell-specific surface glycoprotein CD28(CD28) is a single-pass typeI membrane protein which contains one Ig-likeV-type (immunoglobulin-like) domain. It belongs to the immunoglobulin(Ig) superfamily. CD28 is one of the molecules expressed on T cells that provide co-stimulatory signals, which are required for T cell activation.CD28 co-stimulation is necessary for CD4 positive T-cell proliferation and survival, interleukin-2 production, and T-helper type-2 development. Human post-thymic regulatory T cells require CD28 co-stimulation to expand and maintain potent suppressive function in vivo. Apoptosis plays a key role in the age-related decline of CD28 expression and in immunosenescence. CD28 is the receptor for CD80 (B7.1) and CD86 (B7.2). When activated by Toll-like receptor ligands, the CD80 expression is upregulated in antigen presenting cells (APCs). The CD86 expression on antigen presenting cells is constitutive. CD28 is the only B7 receptor constitutively expressed on naive T cells.
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