CD28 Protein, Human, Recombinant (hFc & Avi), Biotinylated

CD28 Protein, Human, Recombinant (hFc & Avi), Biotinylated is expressed in HEK293 mammalian cells with hFc and Avi tag. The predicted molecular weight is 43.7 kDa and the accession number is P10747-1.

CD28 Protein, Human, Recombinant (hFc & Avi), Biotinylated

パッケージサイズ	在庫状況	単価(税別)
20 μg	在庫あり	￥ 54,000
100 μg	約5 days	￥ 191,500
200 μg	約5 days	￥ 326,000
500 μg	約5 days	￥ 659,000

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生物学的情報	Testing in progress
説明	CD28 Protein, Human, Recombinant (hFc & Avi), Biotinylated is expressed in HEK293 mammalian cells with hFc and Avi tag. The predicted molecular weight is 43.7 kDa and the accession number is P10747-1.

Species	Human
Expression Host	HEK293 Cells
Tag	hFc, Avi
Accession Number	P10747-1
別名	CD28 molecule, Tp44
Construction	The Human CD28 (NP_006130.1) (Met1-Pro152) was expressed with a C-terminal Fc region of Human IgG1 tag followed by an AVI tag. The expressed protein was biotinylated in vivo by the Biotin-Protein ligase (BirA enzyme) which is co-expressed. Human, Cynomolgus and Rhesus CD28 sequences (Asn19-Pro152) are identical.
Protein Purity	≥ 95 % as determined by SDS-PAGE. ≥ 90 % as determined by SEC-HPLC.
分子量	43.7 kDa (predicted)

Endotoxin	< 1.0 EU/μg of the protein as determined by the LAL method.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
Reconstitution	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
Stability & Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted proteinsolutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
Shipping	In general, Lyophilized powders are shipping with blue ice.
Research Background	CD28 (Cluster of Differentiation 28) is a disulphide-bonded glycoprotein belonging to the immunoglobulin (Ig) superfamily, and structurally consists of a single Ig V-like extracellular domain, a transmembrane domain and an intracellular domain. Mouse CD28 is constitutively expressed on the surface of all murine T cells and on developing thymocytes as disulfide-linked homodimers or as monomers. CD28 can binds the B7-1 and B7-2 ligand, and together perform important functions in the T and B cell response pathways. B7/CD28 family members, which can augment or antagonize T-cell receptor signaling, in the regulation of central and peripheral T-cell tolerance. CD28 is thus involved in T-cell activation, the induction of cell proliferation and cytokine production and promotion of T-cell survival.Cancer ImmunotherapyCo-stimulatory Immune Checkpoint TargetsImmune CheckpointImmune Checkpoint Detection: AntibodiesImmune Checkpoint Detection: ELISA AntibodiesImmune Checkpoint Detection: FCM AntibodiesImmune Checkpoint Detection: IHC AntibodiesImmune Checkpoint Detection: WB AntibodiesImmune Checkpoint TargetsImmunotherapyTargeted Therapy

参考文献

1. Keir ME,et al.(2005) The B7/CD28 costimulatory family in autoimmunity. Immunol Rev. 204: 128-43. 2. Sansom DM,et al.(2006) The role of CD28 and cytotoxic T-lymphocyte antigen-4 (CTLA-4) in regulatory T-cell biology. Immunol Rev. 212: 131-48. 3. Bjrgo E,et al.(2010) Novel mechanism of signaling by CD28. Immunol Lett. 129(1): 1-6.

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CD28 Protein, Human, Recombinant (hFc & Avi), Biotinylated

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