CD299 Protein, Human, Recombinant

CD299 Protein, Human, Recombinant is expressed in HEK293 mammalian cells. The predicted molecular weight is 37 kDa and the accession number is Q9H2X3-1.

CD299 Protein, Human, Recombinant

パッケージサイズ	在庫状況	単価(税別)
50 μg	約5 days	￥ 114,500

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説明	CD299 Protein, Human, Recombinant is expressed in HEK293 mammalian cells. The predicted molecular weight is 37 kDa and the accession number is Q9H2X3-1.

Species	Human
Expression Host	HEK293 Cells
Tag	Tag Free
Accession Number	Q9H2X3-1
別名	HP10347, CLEC4M, DCSIGNR, MGC47866, L-SIGN, C-type lectin domain family 4 member M, LSIGN, DC-SIGNR, DC-SIGN2, MGC129964, CD209L, CD299
Construction	Human CLEC4M (NP_055072.3) (Ser78-Glu399)
Protein Purity	> 80 % as determined by SDS-PAGE
分子量	37 kDa (predicted)

Endotoxin	< 1.0 EU/μg of the protein as determined by the LAL method.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
Reconstitution	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
Stability & Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted proteinsolutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
Shipping	In general, Lyophilized powders are shipping with blue ice.
Research Background	C-type lectin domain family 4, member M, also known as DC-SIGNR and CLEC4M, is a type II integral membrane protein that is 77% amino acid identical to DC-SIGN, an HIV gp120-binding protein. Though the encoded gene located in the same chromosome, DC-SIGN is expressed solely on dendritic cells, while DC-SIGNR is predominantly found in liver sinusoidal endothelial cells and lymph node, as well as placental endothelium. DC-SIGNR exists as a homotetramer, and the tandem repeat domain, also called neck domain, mediates oligomerization. DC-SIGNR is regarded as a pathogen-recognition receptor involved in peripheral immune surveillance in liver, and probably mediates the endocytosis of pathogens which are subsequently degraded in lysosomal compartments. DC-SIGNR appears to selectively recognize and bind many viral surface glycoproteins containing high mannose N-linked oligosaccharides in a calcium-dependent manner, including HIV-1 gp120, HIV-2 gp120, SIV gp120, ebolavirus glycoproteins, HCV E2, and human SARS coronavirus protein S, as well as the cellular adhesion protein ICAM3. DC-SIGNR has been thought to play an important role in establishing HIV infection by enhancing trans-infection of CD4(+)T cells in the regional lymph nodes. It may affect susceptibility to HIV infection by a mechanism that is different in females and males. DC-SIGNR can bind to hepatitis C virus (HCV), and its polymorphism might affect HCV loads supporting the concept that DC-SIGNR contributes to HCV replication efficacy.

参考文献

1. Nattermann J,et al.(2006) The tandem-repeat polymorphism of the DC-SIGNR gene in HCV infection. J Viral Hepat. 13(1): 42-6. 2. Wichukchinda N,et al.(2007) The polymorphisms in DC-SIGNR affect susceptibility to HIV type 1 infection. AIDS Res Hum Retroviruses. 23(5): 686-92.

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CD299 Protein, Human, Recombinant

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