CD32B/Fcgr2b Protein, Cynomolgus, Recombinant (His & Avi), Biotinylated is expressed in HEK293 mammalian cells with His and Avi tag. The predicted molecular weight is 23.5 kDa and the accession number is Q8SPW3.
| 生物学的情報 |
Immobilized Cynomolgus CD32B/Fcgr2b Protein (His & AVI Tag), Biotinylated at 2 μg/mL (100 μL/well) can bind Human IgG2 Protein (257 Ser/Ala) , the EC50 is 4-36 ng/mL.
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| 説明 |
CD32B/Fcgr2b Protein, Cynomolgus, Recombinant (His & Avi), Biotinylated is expressed in HEK293 mammalian cells with His and Avi tag. The predicted molecular weight is 23.5 kDa and the accession number is Q8SPW3. |
| Species |
Cynomolgus
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| Expression Host |
HEK293 Cells
|
| Tag |
His, Avi
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| Accession Number |
Q8SPW3
|
| 別名 |
Fc fragment of IgG, low affinity IIb, receptor (CD32)
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| Construction |
The Cynomolgus (Macaca fascicularis) CD32b (AAL92097.1) extracellular domain (Met 1-Gly 220) was fused with a c-terminal polyhistidine tagged AVI tag at the C-terminus. The expressed protein was biotinylated in vivo by the Biotin-Protein ligase (BirA enzyme) which is co-expressed.
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| Protein Purity |
> 97 % as determined by SDS-PAGE
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| 分子量 |
23.5 kDa (predicted)
|
| Endotoxin |
< 1.0 EU/μg of the protein as determined by the LAL method.
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| Formulation |
Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
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| Reconstitution |
A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
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| Stability & Storage |
It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted proteinsolutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
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| Shipping |
In general, Lyophilized powders are shipping with blue ice.
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