CD32B/Fcgr2b Protein, Human, Recombinant (His & Avi), Biotinylated is expressed in HEK293 mammalian cells with His and Avi tag. The predicted molecular weight is 24 kDa and the accession number is P31994-2.
| 生物学的情報 |
Loaded Biotinylated Human FcγRIIB/C / CD32b/c protein (His & Avi Tag) on SA Biosensor, can bind Bevacizumab (IgG1) with an affinity constant of 1.2 μM as determined in a BLI assay (QC tested).
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| 説明 |
CD32B/Fcgr2b Protein, Human, Recombinant (His & Avi), Biotinylated is expressed in HEK293 mammalian cells with His and Avi tag. The predicted molecular weight is 24 kDa and the accession number is P31994-2. |
| Species |
Human
|
| Expression Host |
HEK293
|
| Tag |
His,AVI
|
| Accession Number |
P31994-2
|
| 別名 |
FCGR2, FCGR21, CD32A, FCG2, CDw32, CD32B, FcGR, FCGR2A, CD32, Fc gamma RIIB, Fc γ RIIB, IGFR2, Fc gamma RII, Fc fragment of IgG, low affinity IIb, receptor (CD32), Fc γ RII
|
| Construction |
A DNA sequence encoding the extracellular domain (Ala 46-Ile 224) of mature human CD32b (NP_001002274.1) protein was fused with a signal peptide at the N-terminus and a c-terminal polyhistidine tagged AVI tag at the C-terminus. The expressed protein was biotinylated in vivo by the Biotin-Protein ligase (BirA enzyme) which is co-expressed.
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| Protein Purity |
> 95 % as determined by SDS-PAGE. > 90 % as determined by SEC-HPLC
|
| 分子量 |
24 kDa (predicted)
|
| Endotoxin |
< 1.0 EU per μg of the protein as determined by the LAL method.
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| Formulation |
Lyophilized from sterile PBS, pH 7.4. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0. 01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
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| Reconstitution |
A hardcopy of datasheet with reconstitution instructions is sent along with the products. Please refer to it for detailed information.
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| Stability & Storage |
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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| Shipping |
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
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