CD39 Protein, Cynomolgus, Rhesus, Recombinant (His)

CD39 Protein, Cynomolgus, Rhesus, Recombinant (His) is expressed in Baculovirus insect cells with His tag. The predicted molecular weight is 51.8 kDa and the accession number is XP_015311944.1&XP_015003298.1.

CD39 Protein, Cynomolgus, Rhesus, Recombinant (His)

パッケージサイズ	在庫状況	単価(税別)
50 μg	在庫あり	￥ 73,500
100 μg	約5 days	￥ 130,500
200 μg	約5 days	￥ 229,500
500 μg	約5 days	￥ 486,500

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生物学的情報	Measured by its ability to hydrolyze the 5’phosphategroups from the substrate adenosine 5’triphosphate(ATP). The specific activity is > 5000 pmoles/min/μg.
説明	CD39 Protein, Cynomolgus, Rhesus, Recombinant (His) is expressed in Baculovirus insect cells with His tag. The predicted molecular weight is 51.8 kDa and the accession number is XP_015311944.1&XP_015003298.1.

Species	Cynomolgus,Rhesus
Expression Host	Baculovirus Insect Cells
Tag	C-His
Accession Number	XP_015311944.1&XP_015003298.1
別名	ectonucleoside triphosphate diphosphohydrolase 1
Construction	The cynomolgus / rhesus ENTPD1 (XP_015311944.1/XP_015003298.1) (Thr60-Val500) was expressed with a polyhistidine tag at the C-terminus. Cynomolgus and Rhesus ENTPD1 sequences are identical.
Protein Purity	> 90 % as determined by SDS-PAGE.
分子量	51.8 kDa (predicted)

Endotoxin	< 1.0 EU/μg of the protein as determined by the LAL method.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing 20 mM Tris, 300 mM NaCl, 10% glycerol, pH 7.5. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
Reconstitution	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
Stability & Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted proteinsolutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
Shipping	In general, Lyophilized powders are shipping with blue ice.
Research Background	CD39, also known as ENTPD1, belongs to the GDA1/CD39 NTPase family. It is expressed primarily on activated lymphoid cells and can also be detected in endothelial tissues. The vascular isoform and the placental isoform II are present in both placenta and umbilical vein, whereas placental isoform I is present in placenta only. CD39 can hydrolyze both nucleoside triphosphates and diphosphates. It is the dominant ecto nucleotidase of vascular and placental trophoblastic tissues and appears to modulate the functional expression of type 2 purinergic (P2) G protein coupled receptors (GPCRs). CD39 transgenic mice exhibit impaired platelet aggregation, prolonged bleeding times, and resistance to systemic thromboembolism. There is a correlation between ATP hydrolysis and triglycerides in patients with chronic heart disease, suggesting a relationship between ATP diphosphohydrolase and thrombogenesis. In the nervous system, CD39 could hydrolyze ATP and other nucleotides to regulate purinergic neurotransmission.

参考文献

1. Kunzli BM,et al. (2011) Variable impact of CD39 in experimental murine colitis. Dig Dis Sci. 2011 56 (5): 1393-403. 2. Clayton A,et al. (2011) Cancer exosomes express CD39 and CD73, which suppress T cells through adenosine production. J Immunol. 187 (2): 676-83. 3. Loza MJ,et al. (2011) T-cell specific defect in expression of the NTPDase CD39 as a biomarker for lupus. Cell Immunol. 271 (1): 110-7.

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CD39 Protein, Cynomolgus, Rhesus, Recombinant (His)

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