CD3D & CD3E Heterodimer Protein, Human, Recombinant (Flag & His) is expressed in HEK293 mammalian cells with Flag and His tag. The predicted molecular weight is 31.05 kDa and the accession number is P04234-1&P07766-1.
パッケージサイズ | 在庫状況 | 単価(税別) |
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50 μg | 在庫あり | ¥ 88,500 |
100 μg | 約5 days | ¥ 156,500 |
200 μg | 約5 days | ¥ 275,500 |
500 μg | 約5 days | ¥ 583,000 |
生物学的情報 |
Measured by its binding ability in a functional ELISA. Immobilized human CD3D & CD3E Heterodimer Protein at 2 μg/ml (100 μl/well) can bind OKT3-mIgG2a . The EC50 of OKT3-mIgG2a is 7-28 ng/ml.
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説明 | CD3D & CD3E Heterodimer Protein, Human, Recombinant (Flag & His) is expressed in HEK293 mammalian cells with Flag and His tag. The predicted molecular weight is 31.05 kDa and the accession number is P04234-1&P07766-1. |
Species | Human |
Expression Host | HEK293 Cells |
Tag | Flag, His |
Accession Number | P04234-1&P07766-1 |
別名 | CD3-DELTA, CD3d molecule, delta (CD3-TCR complex), CD3d molecule, δ (CD3-TCR complex), CD3-δ, IMD19, T3D |
Construction | The extracellular domain (Met1-Ala105) of Human CD3D (P04234-1) was fused with a flag tag at the C-terminus, constructed the plasmid 1; The extracellular domain (Met1-Asp126) of Human CD3E (P07766-1) was fused with a polyhistidine tag at the C-terminus, constructed the plasmid 2. The two plasmids were co-expressed and the Human CD3D/CD3E heterodimer was purified. |
Protein Purity |
≥ 95 % as determined by SDS-PAGE. ≥ 95 % as determined by SEC-HPLC.
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分子量 | 31.05 kDa (predicted) |
Endotoxin | < 1.0 EU/μg of the protein as determined by the LAL method. |
Formulation | Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization. |
Reconstitution | A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information. |
Stability & Storage |
It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted proteinsolutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
Shipping |
In general, Lyophilized powders are shipping with blue ice. |
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Please read the User Guide of Recombinant Proteins for more specific information.
CD3D & CD3E Heterodimer Protein, Human, Recombinant (Flag & His) CD3-DELTA CD3d molecule, delta (CD3-TCR complex) CD3d molecule, δ (CD3-TCR complex) CD3d molecule, delta CD3-δ IMD 19 CD3-TCR complex IMD19 T3D IMD-19 recombinant recombinant-proteins proteins protein