CD3D & CD3E Heterodimer Protein, Human, Recombinant is expressed in HEK293 mammalian cells. The predicted molecular weight is 77.5 kDa and the accession number is P04234-1&P07766-1.
| 生物学的情報 |
Measured by its ability to bind OKT3-mIgG2a in a functional ELISA.
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| 説明 |
CD3D & CD3E Heterodimer Protein, Human, Recombinant is expressed in HEK293 mammalian cells. The predicted molecular weight is 77.5 kDa and the accession number is P04234-1&P07766-1. |
| Species |
Human
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| Expression Host |
HEK293 Cells
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| Tag |
Tag Free
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| Accession Number |
P04234-1&P07766-1
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| 別名 |
CD3d molecule, δ (CD3-TCR complex), CD3-δ, CD3d molecule, delta (CD3-TCR complex), CD3-DELTA, IMD19, T3D
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| Construction |
The extracellular domain (Met1-Ala105) of Human CD3D (P04234-1) was fused with the C-terminal his-tagged Fc region of Human IgG1 at the C-terminus, constructed the plasmid 1; The extracellular domain (Met1-Asp126) of Human CD3E (P07766-1) was fused with the C-terminal flag-tagged Fc region of Human IgG1 at the C-terminus, constructed the plasmid 2. The two plasmids were co-expressed and the Human CD3D/CD3E heterodimer was purified.
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| Protein Purity |
≥ 90 % as determined by SDS-PAGE. ≥ 95 % as determined by SEC-HPLC.
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| 分子量 |
77.5 kDa (predicted)
|
| Endotoxin |
< 1.0 EU/μg of the protein as determined by the LAL method.
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| Formulation |
Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
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| Reconstitution |
A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
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| Stability & Storage |
It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted proteinsolutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
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| Shipping |
In general, Lyophilized powders are shipping with blue ice.
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