CD3D & CD3E Heterodimer Protein, Rhesus, Recombinant (Fc-Flag & Fc-His) is expressed in HEK293 mammalian cells with Fc-Flag, Fc-His tag. The predicted molecular weight is 76.63 kDa and the accession number is F6WI60-1.
| 説明 |
CD3D & CD3E Heterodimer Protein, Rhesus, Recombinant (Fc-Flag & Fc-His) is expressed in HEK293 mammalian cells with Fc-Flag, Fc-His tag. The predicted molecular weight is 76.63 kDa and the accession number is F6WI60-1. |
| Species |
Rhesus
|
| Expression Host |
HEK293 Cells
|
| Tag |
Fc-Flag, Fc-His
|
| Accession Number |
F6WI60-1
|
| 別名 |
CD3d molecule, delta (CD3-TCR complex), CD3d molecule, δ (CD3-TCR complex)
|
| Construction |
The Rhesus CD3D (F6WI60-1)(Met1-Ala105) was fused with the C-terminal flag-tagged Fc region of Human IgG1 at the C-terminus, constructed the plasmid 1; The Rhesus CD3E (XP_014971302.1) (Met1-Asp117) with the C-terminal his-tagged Fc region of Human IgG1 at the C-terminus, constructed the plasmid 2. The two plasmids were co-expressed and the CD3D&CD3E heterodimer was purified.
|
| Protein Purity |
> 95 % as determined by SDS-PAGE.
|
| 分子量 |
76.63 kDa (predicted)
|
| Endotoxin |
< 1.0 EU/μg of the protein as determined by the LAL method.
|
| Formulation |
Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
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| Reconstitution |
A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
|
| Stability & Storage |
It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted proteinsolutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
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| Shipping |
In general, Lyophilized powders are shipping with blue ice.
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