CD3E & CD3G Heterodimer Protein, Human, Recombinant (Fc-Flag & Fc-His) is expressed in HEK293 mammalian cells with Fc-Flag, Fc-His tag. The predicted molecular weight is 78.36 kDa and the accession number is NP_000724.1.
| 説明 |
CD3E & CD3G Heterodimer Protein, Human, Recombinant (Fc-Flag & Fc-His) is expressed in HEK293 mammalian cells with Fc-Flag, Fc-His tag. The predicted molecular weight is 78.36 kDa and the accession number is NP_000724.1. |
| Species |
Human
|
| Expression Host |
HEK293 Cells
|
| Tag |
C-hFc-Flag, C-hFc-His
|
| Accession Number |
NP_000724.1
|
| 別名 |
CD3e molecule, epsilon (CD3-TCR complex), TCRE, CD3e molecule, ε (CD3-TCR complex), T3E, IMD18, CD3E & CD3G Heterodimer
|
| Construction |
The (Met1-Asp126) of Human CD3E (NP_000724.1) was fused with a C-terminal Fc region of Human IgG1 tag followed by a FLAG tag, constructed the plasmid 1; The (Met1-Ser116) of Human CD3G (NP_000064.1) was fused with a C-terminal Fc region of Human IgG1 tag followed by a polyhistidine tag, constructed the plasmid 2. The two plasmids were co-expressed and the Human CD3E/CD3G heterodimer was purified.
|
| Protein Purity |
≥ 95 % as determined by SDS-PAGE. ≥ 95 % as determined by SEC-HPLC.
|
| 分子量 |
78.36 kDa (predicted)
|
| Endotoxin |
< 1.0 EU/μg of the protein as determined by the LAL method.
|
| Formulation |
Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
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| Reconstitution |
A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
|
| Stability & Storage |
It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted protein solutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
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| Shipping |
In general, Lyophilized powders are shipping with blue ice.
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