CD3E & CD3G Heterodimer Protein, Mouse, Recombinant (hFc) is expressed in HEK293 mammalian cells with hFc tag. The predicted molecular weight is 74.5 kDa and the accession number is NP_031674.1&NP_033980.1.
| 説明 |
CD3E & CD3G Heterodimer Protein, Mouse, Recombinant (hFc) is expressed in HEK293 mammalian cells with hFc tag. The predicted molecular weight is 74.5 kDa and the accession number is NP_031674.1&NP_033980.1. |
| Species |
Mouse
|
| Expression Host |
HEK293 Cells
|
| Tag |
C-hFc
|
| Accession Number |
NP_031674.1&NP_033980.1
|
| 別名 |
CD3e molecule, epsilon (CD3-TCR complex), CD3ε, AI504783, CD3epsilon, CD3e molecule, ε (CD3-TCR complex), T3e, CD3
|
| Construction |
The extracellular domain (Met1-Asp108) of Mouse CD3E (NP_031674.1) was fused with the Fc region of Human IgG1 at the C-terminus, constructed the plasmid 1; The extracellular domain (Met1-Ser116) of Mouse CD3G (NP_033980.1) was fused with the Fc region of Human IgG1 at the C-terminus, constructed the plasmid 2. The two plasmids were co-expressed and the Mouse CD3E/CD3G heterodimer was purified.
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| Protein Purity |
> 90 % as determined by SDS-PAGE
|
| 分子量 |
74.5 kDa (predicted)
|
| Endotoxin |
< 1.0 EU/μg of the protein as determined by the LAL method.
|
| Formulation |
Lyophilized from a solution filtered through a 0.22 μm filter, containing 20 mM Tris, 150 mM NaCl, 10% Glycerol, 0.02% Tween 20, pH8.5. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
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| Reconstitution |
A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
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| Stability & Storage |
It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted protein solutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
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| Shipping |
In general, Lyophilized powders are shipping with blue ice.
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