CD40 Ligand Protein, Human, Recombinant (His & Avi), Biotinylated is expressed in HEK293 mammalian cells with N-6xHis-Avi tag. The predicted molecular weight is 23-28 KDa and the accession number is P29965.
| 説明 |
CD40 Ligand Protein, Human, Recombinant (His & Avi), Biotinylated is expressed in HEK293 mammalian cells with N-6xHis-Avi tag. The predicted molecular weight is 23-28 KDa and the accession number is P29965. |
| Species |
Human
|
| Expression Host |
HEK293 Cells
|
| Tag |
N-6xHis-Avi
|
| Accession Number |
P29965
|
| 別名 |
CD40 Ligand, TNF-Related Activation Protein, CD40LG, TNFSF5, CD40L, CD40-L, Tumor Necrosis Factor Ligand Superfamily Member 5, CD154, T-Cell Antigen Gp39, TRAP
|
| Amino Acid |
Glu108-Leu261
|
| Construction |
Glu108-Leu261
|
| Protein Purity |
Greater than 95% as determined by reducing SDS-PAGE. (QC verified)
|
| 分子量 |
23-28 KDa (reducing condition)
|
| Endotoxin |
< 0.1 ng/µg (1 EU/µg) as determined by LAL test.
|
| Formulation |
Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, 1 mM EDTA, pH 7.4.
|
| Reconstitution |
Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.
|
| Stability & Storage |
Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted protein solutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
|
| Shipping |
In general, Lyophilized powders are shipping with blue ice. Solutions are shipping with dry ice.
|
| Research Background |
CD40 Ligand (CD40LG) is a type II transmembrane glycoprotein that belongs to the TNF superfamily. Like other TNF superfamily members, CD40LG exists as a trimer in membrane bound and soluble form, both of which are bioactive. CD40LG is a ligand for CD40; its ligation also initiates signal transduction in CD40LG expressing cells. CD40LG is a differentiation antigen that is expressed on the surface of T-cells. It stimulates B-cell proliferation and secretion of all immunoglobulin isotypes in the presence of cytokines. CD40LG has been shown to induce cytokine production and tumoricidal activity in peripheral blood monocytes. It also co-stimulates proliferation of activated T-cells and this is accompanied by the production of IFN-gamma, TNF-alpha, and IL2.
|
Calculator
Reconstitution Calculator
Recombinant Proteins Dilute Calculator
Specific Activity Calculator
bottom
