CD47 Protein, Human, Recombinant (aa 1-139, His)

CD47 Protein, Human, Recombinant (aa 1-139, His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 15.2 kDa and the accession number is Q08722-3.

CD47 Protein, Human, Recombinant (aa 1-139, His)

パッケージサイズ	在庫状況	単価(税別)
100 μg	在庫あり	￥ 88,500
200 μg	約5 days	￥ 156,500
500 μg	約5 days	￥ 330,500

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生物学的情報

1. Measured by its binding ability in a functional ELISA. Immobilized Human SIRP alpha hFc at 2 μg/mL (100 μL/well) can bind Human CD47 His, the EC50 of Human CD47 His is 5.0-25.0 ng/mL.
2. Captured Ligufalimab (IgG4) on proA Chip can bind CD47 with an affinity constant of 10.82 nM as determined in a SPR assay (Routinely tested).
3. Loaded Hu5F9-G4 (IgG4) on ProA Biosensor, can bind human CD47 protein, His Tag with an affinity constant of 3.02 nM as determined in BLI assay (Routinely tested).

説明

CD47 Protein, Human, Recombinant (aa 1-139, His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 15.2 kDa and the accession number is Q08722-3.

Species	Human
Expression Host	HEK293
Tag	His
Accession Number	Q08722-3
別名	MER6, CD47 molecule, IAP, OA3
Construction	A DNA sequence encoding the human CD47 (NP_942088.1) (Met1-Pro139) was expressed with a polyhistidine tag at the C-terminus.
Protein Purity	≥ 95 % as determined by SDS-PAGE. ≥ 95 % as determined by SEC-HPLC.
分子量	15.2 kDa (predicted)

Endotoxin	< 1.0 EU per μg of the protein as determined by the LAL method.
Formulation	Lyophilized from sterile PBS,pH7.4. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0. 01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
Reconstitution	A hardcopy of datasheet with reconstitution instructions is sent along with the products. Please refer to it for detailed information.
Stability & Storage	Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Shipping	In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Research Background	CD47 contains 1 Ig-like V-type (immunoglobulin-like) domain and is a receptor for the C-terminal cell binding domain of thrombospondin. It may play a role in membrane transport and signal transduction. CD47 is also a membrane protein, which is involved in the increase in intracellular calcium concentration that occurs upon cell adhesion to extracellular matrix. It is very broadly distributed on normal adult tissues, as well as ovarian tumors, being especially abundant in some epithelia and the brain. CD47 may play a role in membrane transport and/or integrin dependent signal transduction. It may prevent premature elimination of red blood cells. It also may be involved in membrane permeability changes induced following virus infection. By acting as an adhesion receptor for THBS1 on platelets, CD47 plays a role in both cell adhesion and in the modulation of integrins. It also plays an important role in memory formation and synaptic plasticity in the hippocampus.Cancer ImmunotherapyCo-inhibitory Immune Checkpoint TargetsImmune CheckpointImmune Checkpoint Detection: AntibodiesImmune Checkpoint Detection: ELISA AntibodiesImmune Checkpoint Detection: WB AntibodiesImmune Checkpoint TargetsImmunotherapyTargeted Therapy

参考文献

1. Brown EJ, et al. (2001) Integrin-associated protein (CD47) and its ligands. Trends Cell Biol. 11(3): 130-5. 2. Oldenborg PA. (2004) Role of CD47 in erythroid cells and in autoimmunity. Leuk Lymphoma. 45(7): 1319-27. 3. Kaczorowski DJ, et al. (2007) Targeting CD47: NO limit on therapeutic potential. Circ Res. 100(5): 602-3.

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CD47 Protein, Human, Recombinant (aa 1-139, His)

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