CD59 Protein, Human, Recombinant (His)

CD59 Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 10.2 kDa and the accession number is P13987-1.

CD59 Protein, Human, Recombinant (His)

パッケージサイズ	在庫状況	単価(税別)
100 μg	在庫あり	￥ 88,500
200 μg	約5 days	￥ 156,500
500 μg	約5 days	￥ 330,500

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生物学的情報	Testing in progress
説明	CD59 Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 10.2 kDa and the accession number is P13987-1.

Species	Human
Expression Host	HEK293
Tag	His
Accession Number	P13987-1
別名	MIN2, MAC-IP, G344, EL32, FLJ92039, HRF-20, EJ30, HRF20, MSK21, MGC2354, MIRL, 16.3A5, MIN3, FLJ38134, MACIF, CD59 molecule, p18-20, MIC11, MEM43, EJ16, MIN1, 1F5
Construction	A DNA sequence encoding the mature form of human CD59 (P13987-1) (Met 1-Glu 101) without the pro peptide was fused with a polyhistidine tag at the C-terminus.
Protein Purity	≥ 95 % as determined by SDS-PAGE. ≥ 90 % as determined by SEC-HPLC.
分子量	10.2 kDa (predicted)

Endotoxin	< 1.0 EU per μg of the protein as determined by the LAL method.
Formulation	Lyophilized from sterile PBS, pH 7.4. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0. 01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
Reconstitution	A hardcopy of datasheet with reconstitution instructions is sent along with the products. Please refer to it for detailed information.
Stability & Storage	Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Shipping	In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Research Background	CD59 glycoprotein, also known as 2 kDa homologous restriction factor, HRF2, MAC-inhibitory protein, Membrane attack complex inhibition factor, Membrane inhibitor of reactive lysis, MIC11, MIRL and CD59, is a cell membrane protein which contains one UPAR/Ly6 domain. CD59 is a small, highly glycosylated, GPI-linked protein, with a wide expression profile. The soluble form of CD59 from urine retains its specific complement binding activity, but exhibits greatly reduced ability to inhibit MAC assembly on cell membranes. CD59 is a potent inhibitor of the complement membrane attack complex (MAC) action. CD59 was first identified as a regulator of the terminal pathway of complement. It acts by binding to the C8 and/or C9 complements of the assembling MAC, thereby preventing incorporation of the multiple copies of C9 required for complete formation of the osmolytic pore. This inhibitor appears to be species-specific. CD59 is involved in signal transduction for T-cell activation complexed to a protein tyrosine kinase. Defects in CD59 are the cause of CD59 deficiency (CD59D).

参考文献

1. Fletcher CM. et al., 1994, Structure. 2: 185-99. 2. Rudd PM. et al., 1997, J Biol Chem. 272: 7229-44. 3. Kimberley FC. et al., 2007, Mol Immunol. 44 (1-3): 73-81. 4. Gong Y. et al., 2007, Sci China C Life Sci. 50 (6): 773-9. 5. Picariello G. et al., 2008, Proteomics 8: 3833-47. 6. Heibeck TH. et al., 2009, J Proteome Res. 8: 3852-61.

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CD59 Protein, Human, Recombinant (His)

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